Model-guided Discovery and Optimization of Cell-based Sensors
This is a MURI project led by Chris Voigt at MIT and involving Domitilla Del Vecchio (MIT), Michael Laub (MIT), Vincent Noireaux (UMN), Eduardo Sontag (Rutgers), Howard Salis (Penn State) and Jeff Tabor (Rice). The information on this page focuses primarily on the work involving my research group.
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Current participants: Additional participants: Past participants:
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Objectives
We are applying tools from synthetic biology to construct high-performance and robust sensors that respond to non-natural signals. Our collaborators are focused on the design of sensors for the non-visible light spectrum (UV and IR) and magnetic fields, including the use of discovery methods to build first-generation genetic sensors. In practice, while these synthetic sensors are responsive under lab conditions, they lack the performance, reliability, and environmental robustness necessary for in-field applications. To this end, we are applying tools from control theory and a new concept for the in vitro characterization of genetic devices (“breadboarding”) to develop parts and design principles that make the sensors robust to environment, genetic context, and host.
- Task 3.1: Analyze the robustness of alternative sensors designs that incorporate positive and negative feedback loops
- Task 3.2: Quantify the impact of feedback loop timescales on sensor robustness
- Task 3.3: Determine the design principles for robustness of of phosphorelay circuits to changes in the concentration of ATP and AcetylBP.
References
- Systematic Design and Implementation of a Novel Synthetic Fold-Change Detector Biocircuit In Vivo. Shaobin Guo, Yutaka Hori, and Richard M Murray. Submitted, 2015 Winter q-bio Conference (5 Nov 2014).
- System identification of phosophorylation based insulator in a cell-free in vitro transcription-translation system. Enoch Yeung, Shaobin Guo, Richard M. Murray. Submitted, 2014 Winter q-bio Conference (5 Nov 2013).
- Implementation And Simulation Of Phosphorylation-Based Insulator In Transcription-Translation Platform. Shaobin Guo, Enoch Yeung, Kayzad Soli Nilgiriwala, Domitilla Del Vecchio, Richard M. Murray. Submitted, 2014 Winter q-bio Conference (5 Nov 2013).
- Implementation And Simulation Of Phosphorylation-Based Insulator In Transcription-Translation Platform, Shaobin Guo, Enoch Yeung, Kayzad Soli Nilgiriwala, Domitilla Del Vecchio, Richard M. Murray. Submitted, 2014 Winter q-bio Conference (5 Nov 2013).
- System identification of phosophorylation based insulator in a cell-free in vitro transcription-translation system, Enoch Yeung, Shaobin Guo, Richard M. Murray. Submitted, 2014 Winter q-bio Conference (5 Nov 2013).
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