Developing Standardized Cell-Free Platforms for Rapid Prototyping of Synthetic Biology Circuits and Pathways

From Murray Wiki
(Redirected from NSF Cell Free)
Jump to navigationJump to search

The goal of this project is to further advance standardized cell-free systems from engineered E. coli and other organisms for use in prototyping synthetic circuit and pathway designs. Such standardized systems will both explore the boundaries of cell-free prototyping and characterization, and enable more detailed understanding of key mechanisms, accelerating the usage and broader utility of cell-free systems in industry and academia. The long term vision for this project is to establish cell-free systems as a platform for implementation of synthetic biological circuits, pathways, and systems, where modular and complex biomolecular systems can be engineered in a systematic fashion. This project seeks to overcome some of the current limitations of cell-free systems through a combination of experimental characterization and computational modeling.

Current participants:

Additional participants:


  • Paul Freemont (Imperial College London)

Past participants:



The main objectives of this project are:

  • Development of well-understood, standardized TX-TL reaction systems that are suitable for prototyping circuits and pathways for a variety of cells
  • Characterization and modeling of complex synthetic biology components, circuits, and pathways using TX-TL that enable forward engineering
  • Development of new biochemical indicator components for use in TX-TL systems to achieve better understanding and more predictive models


  • Agency: NSF
  • Grant number: CBET-1903477
  • Start date: 1 Jul 2019
  • End date: 30 Jun 2023
  • Support: 1 graduate student
  • Reporting: Annual reports