Difference between revisions of "Design of Digitally Controlled Bacterial Circuits for Bioenabled Materials"

From Murray Wiki
Jump to navigationJump to search
 
(10 intermediate revisions by the same user not shown)
Line 16: Line 16:
  
 
=== Objectives ===
 
=== Objectives ===
[[Image:project-name.png|right|400px]]
+
[[Image:arl20-elm.png|right|400px]]
Description of the main objectives of the project
+
The work taking place at Caltech is focused on amplification of redox signal via long-distance, pulsatile cell-cell signaling. Cells embedded in a solid media will have diffusion-limited access to molecules oxidized or reduced at the electrode surface. To achieve uniform response to electrical signals, cells nearer to the electrode must communicate with distant cells. We will augment the redox-sensitive cells with a long-distance signaling circuit, previously studied by our lab, to promote a uniform bacterial reaction to electrical input.
 +
 
 +
Specific objectives under this task:
 +
* Modify the synthetic SoxR/S regulon such that the output protein is Cmi, the signal synthaseproteinoftheP.aeruginosaCm quorumsensingsystem.
 +
* Demonstrate that the redox-sensitive cells can initiate a traveling pulse of cell-cell signaling activity that moves through cells embedded in a hydrogel to increase the population of cells activated in response to electrical input.
  
 
=== References ===
 
=== References ===
 
{{project paper list}}
 
{{project paper list}}
  
[[Category:Pending project]]
+
[[Category:Completed projects]]
<!-- [[Category:Subgroup projects]] -->
+
[[Category:Biocircuits projects]]
 
{{Project
 
{{Project
|Title=In silico-biological control of bacterial activity in composite materials using Scalable Assembly Processes
+
|Title=Design of Digitally Controlled Bacterial Circuits for Bioenabled Materials
|Agency=Army Research Lab/Institute for Collaborative Biotechnology
+
|Agency=Army Research Lab/Institute for Collaborative Biotechnologies
 +
|Grant number=W911NF-19-D-0001/KK1956 (Task 6)
 
|Start date=1 Oct 2019
 
|Start date=1 Oct 2019
 
|End date=30 Sep 2021
 
|End date=30 Sep 2021
 
|Support summary=1 graduate student
 
|Support summary=1 graduate student
 
|Reporting requirements=Annual report
 
|Reporting requirements=Annual report
 +
|Project ID=ICB Materials
 +
|ack=This research is supported by the Institute for Collaborative Biotechnologies through contract W911NF-19-D-0001 from the U.S. Army Research Office. The content of the information on this page does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred.
 
}}
 
}}

Latest revision as of 02:42, 4 August 2021

This proposal seeks to understand if bacteria can be designed to perform on demand when inside of a composite material. Bacteria provide a rich source that can be exploited to develop novel and cost-effective adaptive systems that could be superior to traditional materials. Critical to this concept is the ability to control bacterial activity inside materials. To explore this, we will test the hypothesis that if bacteria can be designed to respond to weak currents and survive in UV-activated polymers, then combining those bacteria with nanomaterials in a UV-activated polymer will create a material where the activity of embedded bacteria can be controlled using a computer interface. The hypothesis will be tested by engineering circuits in bacteria that are responsive to oxidation/reduction at the surface of nanowires. We will then use scalable assembly processes to combine bacteria and nanowires in mixed UV-activated polymers to create rigid/soft composite material. Composite bacterial nanowire materials will be interfaced with computer controllers to examine manipulation of bacterial activity in the composite materials.

Current participants:

Additional participants:

Collaborators:

  • UCSB

Past participants:

  • James Parkin (PhD student, BE)

Objectives

Arl20-elm.png

The work taking place at Caltech is focused on amplification of redox signal via long-distance, pulsatile cell-cell signaling. Cells embedded in a solid media will have diffusion-limited access to molecules oxidized or reduced at the electrode surface. To achieve uniform response to electrical signals, cells nearer to the electrode must communicate with distant cells. We will augment the redox-sensitive cells with a long-distance signaling circuit, previously studied by our lab, to promote a uniform bacterial reaction to electrical input.

Specific objectives under this task:

  • Modify the synthetic SoxR/S regulon such that the output protein is Cmi, the signal synthaseproteinoftheP.aeruginosaCm quorumsensingsystem.
  • Demonstrate that the redox-sensitive cells can initiate a traveling pulse of cell-cell signaling activity that moves through cells embedded in a hydrogel to increase the population of cells activated in response to electrical input.

References

None to date


This research is supported by the Institute for Collaborative Biotechnologies through contract W911NF-19-D-0001 from the U.S. Army Research Office. The content of the information on this page does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred.

  • Agency: Army Research Lab/Institute for Collaborative Biotechnologies
  • Grant number: W911NF-19-D-0001/KK1956 (Task 6)
  • Start date: 1 Oct 2019
  • End date: 30 Sep 2021
  • Support: 1 graduate student
  • Reporting: Annual report