Theoretical Design of Paradoxical Signaling-Based Synthetic Population Control Circuit in E. coli
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| Title | Theoretical Design of Paradoxical Signaling-Based Synthetic Population Control Circuit in E. coli |
|---|---|
| Authors | Michaëlle N. Mayalu and Richard M. Murray |
| Source | 2020 Winter q-bio |
| Abstract | We have developed a mathematical framework to analyze the cooperative control of cell population homeostasis via paradoxical signaling in synthetic contexts. Paradoxical signaling functions through quorum sensing (where cells produce and release a chemical signal as a function of cell density). Precisely, the same quorum sensing signal provides both positive (proliferation) and negative (death) feedback in different signal concentration regimes. As a consequence, the relationship between intercellular quorum sensing signal concentration and net growth rate (cell proliferation minus death rates) can be non-monotonic. This relationship is a condition for robustness to certain cell mutational overgrowths and allows for increased stability in the presence of environmental perturbations. Here, we explore stability and robustness of a conceptualized synthetic circuit. Furthermore, we asses possible design principles that could exist among a subset of paradoxical circuit implementations. This analysis sparks the development a bio-molecular control theory to identify ideal underlying characteristics for paradoxical signaling control systems. |
| Type | Conference paper |
| URL | https://www.biorxiv.org/content/10.1101/2020.01.27.921734v1 |
| DOI | |
| Tag | mm20-wqbio |
| ID | 2019i |
| Funding | DARPA BioCon |
| Flags | Biocircuits |
