Quantitative characterization of random partitioning in the evolution of plasmid-encoded traits

Title	Quantitative characterization of random partitioning in the evolution of plasmid-encoded traits
Authors	Andrew D. Halleran, Emanuel Flores-Bautista and Richard M. Murray
Source	qBio 2019 Conference
Abstract	Plasmids are found across bacteria, archaea, and eukaryotes and play an important role in evolution. Plasmids exist at different copy numbers, the number of copies of the plasmid per cell, ranging from a single plasmid per cell to hundreds of plasmids per cell. This feature of a copy number greater than one can lead to a population of plasmids within a single cell that are not identical clones of one another, but rather have individual mutations that make a given plasmid unique. During cell division, this population of plasmids is partitioned into the two daughter cells, resulting in a random distribution of different plasmid variants in each daughter. In this study, we use stochastic simulations to investigate how random plasmid partitioning compares to a perfect partitioning model. Our simulation results demonstrate that random plasmid partitioning accelerates mutant allele fixation when the allele is beneficial and the selection is in an additive or recessive regime where increasing the copy number of the beneficial allele results in additional benefit for the host. This effect does not depend on the size of the benefit conferred or the mutation rate, but is magnified by increasing plasmid copy number.
Type	Conference paper
URL	https://www.biorxiv.org/content/10.1101/594879v1
DOI	10.1101/594879
Tag	HFM19-qbio
ID	2019c
Funding	AFOSR BRI
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