Design and application of stationary phase combinatorial promoters
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| Title | Design and application of stationary phase combinatorial promoters |
|---|---|
| Authors | Victoria Hsiao and Aileen Cheng and Richard M. Murray |
| Source | Submitted, 2016 Synthetic Biology: Engineering, Evolution and Design (SEED) Conference |
| Abstract | Current bacterial synthetic circuits rely on the fast dilution and high protein expression that occurs during exponential phase. However, constant exponential phase is both difficult to ensure in a lab environment and almost certainly impractical in any natural setting. Here, we characterize the performance of 13 E. coli native 38 promoters, as well as a previously identified 38 consensus promoter. We then make tetO combinatorial versions of the three strongest promoters to allow for inducible delayed expression. The design of these combinatorial promoters allows for design of circuits with inducible stationary phase activity that can be used for phase-dependent delays in dynamic circuits or spatial partitioning of biofilms. |
| Type | Conference paper |
| URL | http://www.cds.caltech.edu/~murray/preprints/hcm16-seed s.pdf |
| DOI | |
| Tag | hcm16-seed |
| ID | 2016b |
| Funding | ARO ICB |
| Flags | Biocircuits |
