Difference between revisions of "Tunable integrase-mediated differentiation facilitates improved output of burdensome functions in E. coli"

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{{Paper
{{Paper
|Title=Tunable integrase-mediated differentiation facilitates improved output of burdensome functions in E. coli
|Title=Tunable integrase-mediated differentiation facilitates improved output of burdensome functions in E. coli
|Authors=Rory L. Williams, Richard M. Murray
|Authors=R. L. Williams and R. M. Murray
|Source=2019 Synthetic Biology: Engineering, Evolution and Design (SEED) Conference
|Source=bioRxiv
|Abstract=Application of synthetic biology is limited by the capacity of cells to faithfully execute burdensome engineered functions in the face of Darwinian evolution. Division of labor, both metabolic and reproductive, are underutilized in confronting this barrier. To address this, we developed a serine-integrase based differentiation circuit that allows control of the population composition through tuning of the differentiation rate and number of cell divisions differentiated cells can undergo. We applied this system to T7 RNAP-driven expression of a fluorescent protein, and demonstrate both increased duration of circuit function and total production for high burden expression. While T7 expression systems are typically used for high-level short-term expression, this system enables longer duration production, and could be readily applied to burdensome or toxic products not readily produced in bacteria.
|Abstract=Application of synthetic biology is limited by the capacity of cells to faithfully execute burdensome engineered functions in the face of Darwinian evolution. Division of labor, both metabolic and reproductive, are underutilized in confronting this barrier. To address this, we developed a serine-integrase based differentiation circuit that allows control of the population composition through tuning of the differentiation rate and number of cell divisions differentiated cells can undergo. We applied this system to T7 RNAP-driven expression of a fluorescent protein, and demonstrate both increased duration of circuit function and total production for high burden expression. While T7 expression systems are typically used for high-level short-term expression, this system enables longer duration production, and could be readily applied to burdensome or toxic products not readily produced in bacteria.
|URL=https://www.biorxiv.org/content/10.1101/614529v1
|URL=https://www.biorxiv.org/content/10.1101/614529v2.article-info
|Type=Conference paper
|Type=Preprint
|ID=2019b
|ID=2019x
|Tag=WM19-seed
|Tag=WM19-biorxiv
|Funding=ICB Microbial
|Funding=ICB Network19
|DOI=10.1101/614529
|DOI=https://doi.org/10.1101/614529
|Flags=Hide
}}
}}

Revision as of 16:48, 15 June 2021

Title Tunable integrase-mediated differentiation facilitates improved output of burdensome functions in E. coli
Authors R. L. Williams and R. M. Murray
Source bioRxiv
Abstract Application of synthetic biology is limited by the capacity of cells to faithfully execute burdensome engineered functions in the face of Darwinian evolution. Division of labor, both metabolic and reproductive, are underutilized in confronting this barrier. To address this, we developed a serine-integrase based differentiation circuit that allows control of the population composition through tuning of the differentiation rate and number of cell divisions differentiated cells can undergo. We applied this system to T7 RNAP-driven expression of a fluorescent protein, and demonstrate both increased duration of circuit function and total production for high burden expression. While T7 expression systems are typically used for high-level short-term expression, this system enables longer duration production, and could be readily applied to burdensome or toxic products not readily produced in bacteria.
Type Preprint
URL https://www.biorxiv.org/content/10.1101/614529v2.article-info
Tag WM19-biorxiv
ID 2019x
Funding ICB Network19
Flags Hide