Difference between revisions of "Design and implementation of a synthetic biomolecular concentration tracker"
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| authors = Victoria Hsiao, Emmanuel LC de los Santos, Weston R Whitaker, John E Dueber, Richard M Murray | | authors = Victoria Hsiao, Emmanuel LC de los Santos, Weston R Whitaker, John E Dueber, Richard M Murray | ||
| title = Design and implementation of a biomolecular concentration tracker | | title = Design and implementation of a biomolecular concentration tracker | ||
| source = ACS Synthetic Biology ( | | source = ''ACS Synthetic Biology'', 4(2):150–161, 2015 | ||
| year = 2015 | | year = 2015 | ||
| type = | | type = Journal article | ||
| funding = ICB | | funding = ARO ICB 2008 | ||
| url = http://pubs.acs.org/doi/abs/10.1021/sb500024b | | url = http://pubs.acs.org/doi/abs/10.1021/sb500024b | ||
| abstract = | | abstract = | ||
As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this work, we use synthetic protein scaffolds as a modular and tunable mechanism for concentration tracking through negative feedback. Input to the circuit initiates scaffold production, leading to colocalization of a two-component system and resulting in the production of an inhibitory antiscaffold protein. Using a combination of modeling and experimental work, we show that the biomolecular concentration tracker circuit achieves dynamic protein concentration tracking in ''Escherichia coli'' and that steady state outputs can be tuned. | As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this work, we use synthetic protein scaffolds as a modular and tunable mechanism for concentration tracking through negative feedback. Input to the circuit initiates scaffold production, leading to colocalization of a two-component system and resulting in the production of an inhibitory antiscaffold protein. Using a combination of modeling and experimental work, we show that the biomolecular concentration tracker circuit achieves dynamic protein concentration tracking in ''Escherichia coli'' and that steady state outputs can be tuned. | ||
| flags = | | flags = | ||
| tag = hsi+15-ACS | | tag = hsi+15-ACS | ||
| id = | | id = 2013n | ||
}} | }} | ||
Original posted as BioRxiv preprint 10.1101/000448, 15 Nov 2013 | |||
BioRxiv | |||
Latest revision as of 05:21, 10 June 2016
Victoria Hsiao, Emmanuel LC de los Santos, Weston R Whitaker, John E Dueber, Richard M Murray
ACS Synthetic Biology, 4(2):150–161, 2015
As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this work, we use synthetic protein scaffolds as a modular and tunable mechanism for concentration tracking through negative feedback. Input to the circuit initiates scaffold production, leading to colocalization of a two-component system and resulting in the production of an inhibitory antiscaffold protein. Using a combination of modeling and experimental work, we show that the biomolecular concentration tracker circuit achieves dynamic protein concentration tracking in Escherichia coli and that steady state outputs can be tuned.
- Journal article: http://pubs.acs.org/doi/abs/10.1021/sb500024b
- Project(s): ARO ICB 2008
Original posted as BioRxiv preprint 10.1101/000448, 15 Nov 2013
