Difference between revisions of "Design and implementation of a synthetic biomolecular concentration tracker"

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{{HTDB paper
{{HTDB paper
| authors = Victoria Hsiao, Emmanuel LC de los Santos, Weston R Whitaker, John E Dueber, Richard M Murray
| authors = Victoria Hsiao, Emmanuel LC de los Santos, Weston R Whitaker, John E Dueber, Richard M Murray
| title = Design and implementation of a synthetic biomolecular concentration tracker
| title = Design and implementation of a biomolecular concentration tracker
| source = BioRxiv 10.1101/000448, 15 Nov 2013
| source = ''ACS Synthetic Biology'', 4(2):150–161, 2015
| year = 2013
| year = 2015
| type = BioRxiv preprint
| type = Journal article
| funding = ICB
| funding = ARO ICB 2008
| url = http://www.biorxiv.org/content/early/2013/11/15/000448
| url = http://pubs.acs.org/doi/abs/10.1021/sb500024b
| abstract =  
| abstract =  
As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this work, we design, model, and implement a biomolecular concentration tracker, in which an output protein tracks the concentration of an input protein. Synthetic modular protein scaffold domains are used to colocalize a two-component system, and a single negative feedback loop modulates the production of the output protein. Using a combination of model and experimental work, we show that the circuit achieves real-time protein concentration tracking in Escherichia coli and that steady state outputs can be tuned.
As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this work, we use synthetic protein scaffolds as a modular and tunable mechanism for concentration tracking through negative feedback. Input to the circuit initiates scaffold production, leading to colocalization of a two-component system and resulting in the production of an inhibitory antiscaffold protein. Using a combination of modeling and experimental work, we show that the biomolecular concentration tracker circuit achieves dynamic protein concentration tracking in ''Escherichia coli'' and that steady state outputs can be tuned.
 
| flags =  
| flags =  
| tag = hsi+13-biorxiv
| tag = hsi+15-ACS
| id = 2013n
| id = 2013n
}}
}}
Original posted as BioRxiv preprint 10.1101/000448, 15 Nov 2013

Latest revision as of 05:21, 10 June 2016


Victoria Hsiao, Emmanuel LC de los Santos, Weston R Whitaker, John E Dueber, Richard M Murray
ACS Synthetic Biology, 4(2):150–161, 2015

As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this work, we use synthetic protein scaffolds as a modular and tunable mechanism for concentration tracking through negative feedback. Input to the circuit initiates scaffold production, leading to colocalization of a two-component system and resulting in the production of an inhibitory antiscaffold protein. Using a combination of modeling and experimental work, we show that the biomolecular concentration tracker circuit achieves dynamic protein concentration tracking in Escherichia coli and that steady state outputs can be tuned.


Original posted as BioRxiv preprint 10.1101/000448, 15 Nov 2013