Difference between revisions of "Design and implementation of a synthetic biomolecular concentration tracker"
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{{HTDB paper | {{HTDB paper | ||
| authors = Victoria Hsiao, Emmanuel LC de los Santos, Weston R Whitaker, John E Dueber, Richard M Murray | | authors = Victoria Hsiao, Emmanuel LC de los Santos, Weston R Whitaker, John E Dueber, Richard M Murray | ||
| title = Design and implementation of a | | title = Design and implementation of a biomolecular concentration tracker | ||
| source = BioRxiv 10.1101/000448, 15 Nov 2013 | | source = 10.1021/sb500024b, (preprint posted on BioRxiv 10.1101/000448, 15 Nov 2013) | ||
| year = | | year = 2015 | ||
| type = | | type = ACS Synthetic Biology article | ||
| funding = ICB | | funding = ICB | ||
| url = http:// | | url = http://pubs.acs.org/doi/abs/10.1021/sb500024b | ||
| abstract = | | abstract = | ||
As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this work, we | As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this work, we use synthetic protein scaffolds as a modular and tunable mechanism for concentration tracking through negative feedback. Input to the circuit initiates scaffold production, leading to colocalization of a two-component system and resulting in the production of an inhibitory antiscaffold protein. Using a combination of modeling and experimental work, we show that the biomolecular concentration tracker circuit achieves dynamic protein concentration tracking in ''Escherichia coli'' and that steady state outputs can be tuned. | ||
| flags = | | flags = | ||
| tag = hsi+ | | tag = hsi+15-ACS | ||
| id = | | id = 2015n | ||
}} | }} | ||
Revision as of 19:40, 17 May 2016
Victoria Hsiao, Emmanuel LC de los Santos, Weston R Whitaker, John E Dueber, Richard M Murray
10.1021/sb500024b, (preprint posted on BioRxiv 10.1101/000448, 15 Nov 2013)
As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this work, we use synthetic protein scaffolds as a modular and tunable mechanism for concentration tracking through negative feedback. Input to the circuit initiates scaffold production, leading to colocalization of a two-component system and resulting in the production of an inhibitory antiscaffold protein. Using a combination of modeling and experimental work, we show that the biomolecular concentration tracker circuit achieves dynamic protein concentration tracking in Escherichia coli and that steady state outputs can be tuned.
- ACS Synthetic Biology article: http://pubs.acs.org/doi/abs/10.1021/sb500024b
- Project(s): ARO ICB
