Difference between revisions of "Design and implementation of a synthetic biomolecular concentration tracker"

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{{HTDB paper
{{HTDB paper
| authors = Victoria Hsiao, Emmanuel LC de los Santos, Weston R Whitaker, John E Dueber, Richard M Murray
| authors = Victoria Hsiao, Emmanuel LC de los Santos, Weston R Whitaker, John E Dueber, Richard M Murray
| title = Design and implementation of a synthetic biomolecular concentration tracker
| title = Design and implementation of a biomolecular concentration tracker
| source = BioRxiv 10.1101/000448, 15 Nov 2013
| source = 10.1021/sb500024b, (preprint posted on BioRxiv 10.1101/000448, 15 Nov 2013)
| year = 2013
| year = 2015
| type = BioRxiv preprint
| type = ACS Synthetic Biology article
| funding = ICB
| funding = ICB
| url = http://www.biorxiv.org/content/early/2013/11/15/000448
| url = http://pubs.acs.org/doi/abs/10.1021/sb500024b
| abstract =  
| abstract =  
As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this work, we design, model, and implement a biomolecular concentration tracker, in which an output protein tracks the concentration of an input protein. Synthetic modular protein scaffold domains are used to colocalize a two-component system, and a single negative feedback loop modulates the production of the output protein. Using a combination of model and experimental work, we show that the circuit achieves real-time protein concentration tracking in Escherichia coli and that steady state outputs can be tuned.
As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this work, we use synthetic protein scaffolds as a modular and tunable mechanism for concentration tracking through negative feedback. Input to the circuit initiates scaffold production, leading to colocalization of a two-component system and resulting in the production of an inhibitory antiscaffold protein. Using a combination of modeling and experimental work, we show that the biomolecular concentration tracker circuit achieves dynamic protein concentration tracking in ''Escherichia coli'' and that steady state outputs can be tuned.


| flags =  
| flags =  
| tag = hsi+13-biorxiv
| tag = hsi+15-ACS
| id = 2013n
| id = 2015n
}}
}}

Revision as of 19:40, 17 May 2016


Victoria Hsiao, Emmanuel LC de los Santos, Weston R Whitaker, John E Dueber, Richard M Murray
10.1021/sb500024b, (preprint posted on BioRxiv 10.1101/000448, 15 Nov 2013)

As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this work, we use synthetic protein scaffolds as a modular and tunable mechanism for concentration tracking through negative feedback. Input to the circuit initiates scaffold production, leading to colocalization of a two-component system and resulting in the production of an inhibitory antiscaffold protein. Using a combination of modeling and experimental work, we show that the biomolecular concentration tracker circuit achieves dynamic protein concentration tracking in Escherichia coli and that steady state outputs can be tuned.