Difference between revisions of "Design and Implementation of a Biomolecular Circuit for Tracking Protein Concentration"
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| authors = Emmanuel L. C. de los Santos, Victoria Hsiao and Richard M. Murray | | authors = Emmanuel L. C. de los Santos*, Victoria Hsiao* and Richard M. Murray | ||
| title = Design and Implementation of a Biomolecular Circuit for Tracking Protein Concentration | | title = Design and Implementation of a Biomolecular Circuit for Tracking Protein Concentration | ||
| source = 2013 American Control Conference (ACC) | | source = Presented, 2013 American Control Conference (ACC) | ||
| year = 2012 | | year = 2012 | ||
| type = Conference Paper | | type = Conference Paper | ||
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| url = http://www.cds.caltech.edu/~murray/preprints/dhm13-acc.pdf | | url = http://www.cds.caltech.edu/~murray/preprints/dhm13-acc.pdf | ||
| abstract = | | abstract = | ||
This paper describes the design, modeling, synthesis and preliminary validation of a protein concentration regulator circuit. The circuit is designed to maintain the level of a target protein to a reference level, specified by the amount of another protein. This is implemented using a single negative feedback loop that inhibited the production of the target protein once its concentration was equal to the reference amount. A mathematical model consisting of a set of ODEs is derived from mass action laws and Hill function approximations of protein production. Steady-state analysis of the model is used to predict parameter sensitivity and experimental behavior. We implemented this circuit in E. coli using scaffold-based sequestration and transcriptional activation. Preliminary experimental results show the system matching predictions from our model and performing the expected task. | This paper describes the design, modeling, synthesis and preliminary validation of a protein concentration regulator circuit. The circuit is designed to maintain the level of a target protein to a reference level, specified by the amount of another protein. This is implemented using a single negative feedback loop that inhibited the production of the target protein once its concentration was equal to the reference amount. A mathematical model consisting of a set of ODEs is derived from mass action laws and Hill function approximations of protein production. Steady-state analysis of the model is used to predict parameter sensitivity and experimental behavior. We implemented this circuit in ''E. coli'' using scaffold-based sequestration and transcriptional activation. Preliminary experimental results show the system matching predictions from our model and performing the expected task. | ||
| flags = | | flags = | ||
| filetype = PDF | | filetype = PDF | ||
Revision as of 19:44, 17 May 2016
Emmanuel L. C. de los Santos*, Victoria Hsiao* and Richard M. Murray
Presented, 2013 American Control Conference (ACC)
This paper describes the design, modeling, synthesis and preliminary validation of a protein concentration regulator circuit. The circuit is designed to maintain the level of a target protein to a reference level, specified by the amount of another protein. This is implemented using a single negative feedback loop that inhibited the production of the target protein once its concentration was equal to the reference amount. A mathematical model consisting of a set of ODEs is derived from mass action laws and Hill function approximations of protein production. Steady-state analysis of the model is used to predict parameter sensitivity and experimental behavior. We implemented this circuit in E. coli using scaffold-based sequestration and transcriptional activation. Preliminary experimental results show the system matching predictions from our model and performing the expected task.
- Conference Paper: http://www.cds.caltech.edu/~murray/preprints/dhm13-acc.pdf
- Project(s): ARO ICB
