Biomolecular resource utilization in elementary cell-free gene circuits

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Dan Siegal-Gaskins, Vincent Noireaux, and Richard M. Murray
2013 American Control Conference (ACC)

We present a detailed dynamical model of the behavior of transcription-translation circuits in vitro that makes explicit the roles played by essential molecular resources. A set of simple two-gene test circuits operating in a cell-free biochemical âbreadboardâ validate this model and highlight the consequences of limited resource availability. In particular, we are able to confirm the existence of biomolecular âcrosstalkâ and isolate its individual sources. The implications of crosstalk for biomolecular circuit design and function are discussed.