Difference between revisions of "Addressable, “Packet-Based” Intercellular Communication through Plasmid Conjugation"
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Revision as of 23:44, 9 June 2019
|Title||Addressable, “Packet-Based” Intercellular Communication through Plasmid Conjugation|
|Authors||John P. Marken, Richard M. Murray|
|Source||Submitted, 2019 Synthetic Biology: Engineering, Evolution and Design (SEED) Conference|
|Abstract||We develop a system for implementing “packet-based” intercellular communication in an engineered bacterial population via conjugation. Our system uses gRNA-based identification markers that allow messages to be addressed to specific strains via Cas9-mediated cleavage of messages sent to the wrong recipient, which we show reduces plasmid transfer by four orders of magnitude. Integrase-mediated editing of the address on the message plasmid allows cells to dynamically update the message’s recipients in vivo. As a proof-of-concept demonstration of our system, we propose a linear path scheme that would propagate a message sequentially through the strains of a population in a defined order.|