Addressable, “Packet-Based” Intercellular Communication through Plasmid Conjugation
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| Title | Addressable, “Packet-Based” Intercellular Communication through Plasmid Conjugation |
|---|---|
| Authors | John P. Marken and Richard M. Murray |
| Source | Submitted, 2019 Synthetic Biology: Engineering, Evolution and Design (SEED) Conference |
| Abstract | We develop a system for implementing “packet-based” intercellular communication in an engineered bacterial population via conjugation. Our system uses gRNA-based identification markers that allow messages to be addressed to specific strains via Cas9-mediated cleavage of messages sent to the wrong recipient, which we show reduces plasmid transfer by four orders of magnitude. Integrase-mediated editing of the address on the message plasmid allows cells to dynamically update the message’s recipients in vivo. As a proof-of-concept demonstration of our system, we propose a linear path scheme that would propagate a message sequentially through the strains of a population in a defined order. |
| Type | Conference paper |
| URL | https://www.biorxiv.org/content/10.1101/591552v1 |
| DOI | |
| Tag | MM19-seed |
| ID | 2019b |
| Funding | ARO ICB |
| Flags |
