Edit Project: Developing Standardized Cell-Free Platforms for Rapid Prototyping of Synthetic Biology Circuits and Pathways

Project description: {{righttoc}} The goal of this project is to further advance standardized cell-free systems from engineered ''E. coli'' and other organisms for use in prototyping synthetic circuit and pathway designs. Such standardized systems will both explore the boundaries of cell-free prototyping and characterization, and enable more detailed understanding of key mechanisms, accelerating the usage and broader utility of cell-free systems in industry and academia. The long term vision for this project is to establish cell-free systems as a platform for implementation of synthetic biological circuits, pathways, and systems, where modular and complex biomolecular systems can be engineered in a systematic fashion. This project seeks to overcome some of the current limitations of cell-free systems through a combination of experimental characterization and computational modeling. {| cellpadding=0 cellspacing=0 width=80% |- valign=top | Current participants: {{project current participants}} Additional participants: {{project additional participants}} | Collaborators: * Paul Freemont (Imperial College London) Past participants: {{project past participants}} |} === Objectives === [[Image:nsf19-cellfree.png|right|600px]] The main objectives of this project are: * Development of well-understood, standardized TX-TL reaction systems that are suitable for prototyping circuits and pathways for a variety of cells * Characterization and modeling of complex synthetic biology components, circuits, and pathways using TX-TL that enable forward engineering * Development of new biochemical indicator components for use in TX-TL systems to achieve better understanding and more predictive models === References === {{project paper list}} [[Category:Active projects]] [[Category:Biocircuits projects]]

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