View source for Design of Digitally Controlled Bacterial Circuits for Bioenabled Materials

=== Objectives ===
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The work taking place at Caltech is focused on amplification of redox signal via long-distance, pulsatile cell-cell signaling. Cells embedded in a solid media will have diffusion-limited access to molecules oxidized or reduced at the electrode surface. To achieve uniform response to electrical signals, cells nearer to the electrode must communicate with distant cells. We will augment the redox-sensitive cells with a long-distance signaling circuit, previously studied by our lab, to promote a uniform bacterial reaction to electrical input.

Specific objectives under this task:
* Modify the synthetic SoxR/S regulon such that the output protein is Cmi, the signal synthaseproteinoftheP.aeruginosaCm quorumsensingsystem.
* Demonstrate that the redox-sensitive cells can initiate a traveling pulse of cell-cell signaling activity that moves through cells embedded in a hydrogel to increase the population of cells activated in response to electrical input.