View source for Addressable, “Packet-Based” Intercellular Communication through Plasmid Conjugation

{{Paper
|Title=Addressable, “Packet-Based” Intercellular Communication through Plasmid Conjugation
|Authors=John P. Marken,  Richard M. Murray
|Source=qBio 2019 Conference
|Abstract=We develop a system for implementing “packet-based” intercellular communication in an engineered bacterial population via conjugation. Our system uses gRNA-based identification markers that allow messages to be addressed to specific strains via Cas9-mediated cleavage of messages sent to the wrong recipient, which we show reduces plasmid transfer by four orders of magnitude. Integrase-mediated editing of the address on the message plasmid allows cells to dynamically update the message’s recipients in vivo. As a proof-of-concept demonstration of our system, we propose a linear path scheme that would propagate a message sequentially through the strains of a population in a defined order.
|URL=https://www.biorxiv.org/content/10.1101/591552v1
|Type=Conference paper
|ID=2019c
|Tag=MM19-qbio
|Funding=ICB Network19
|DOI=10.1101/591552
}}