Moclo: Difference between revisions

From Murray Wiki
Jump to navigationJump to search
No edit summary
Line 15: Line 15:


{| class="wikitable sortable"
{| class="wikitable sortable"
! Name !! Well !! Type !! Overhangs !! Description !! Link
! Name !! Well !! Type !! Left !! Right !! Description !! Link
|-
|-
| J23100_AB || A1 || Promoter || AB || Weak Constitutive Promoter || https://benchling.com/s/gS6lXC3X/edit
| J23100_AB || A1 || Promoter || A || B || Weak Constitutive Promoter || https://benchling.com/s/gS6lXC3X/edit
|-
|-
| J23100_EB || A2 || Promoter || EB || Weak Constitutive Promoter || https://benchling.com/s/aq8Gxp2O/edit
| J23100_EB || A2 || Promoter || E || B || Weak Constitutive Promoter || https://benchling.com/s/aq8Gxp2O/edit
|-
|-
| J23100_FB || A3 || Promoter || FB || Weak Constitutive Promoter || https://benchling.com/s/rTzq9ToN/edit
| J23100_FB || A3 || Promoter || F || B || Weak Constitutive Promoter || https://benchling.com/s/rTzq9ToN/edit
|-
|-
| J23100_GB || A4 || Promoter || GB || Weak Constitutive Promoter || https://benchling.com/s/5yn5Zy6g/edit
| J23100_GB || A4 || Promoter || G || B || Weak Constitutive Promoter || https://benchling.com/s/5yn5Zy6g/edit
|-
|-
| J23102_AB || A5 || Promoter || AB || Strong Constitutive Promoter || https://benchling.com/s/aQOLd1xr/edit
| J23102_AB || A5 || Promoter || A || B || Strong Constitutive Promoter || https://benchling.com/s/aQOLd1xr/edit
|-
|-
| J23102_EB || A6 || Promoter || EB || Strong Constitutive Promoter || https://benchling.com/s/zOCQC6dM/edit
| J23102_EB || A6 || Promoter || E || B || Strong Constitutive Promoter || https://benchling.com/s/zOCQC6dM/edit
|-
|-
| J23102_FB || A7 || Promoter || FB || Strong Constitutive Promoter || https://benchling.com/s/1pfiY4NG/edit
| J23102_FB || A7 || Promoter || F || B || Strong Constitutive Promoter || https://benchling.com/s/1pfiY4NG/edit
|-
|-
| J23102_GB || A8 || Promoter || GB || Strong Constitutive Promoter || https://benchling.com/s/LmBWThyt/edit
| J23102_GB || A8 || Promoter || G || B || Strong Constitutive Promoter || https://benchling.com/s/LmBWThyt/edit
|-
|-
| J23103_AB || A9 || Promoter || AB || Weak Constitutive Promoter || https://benchling.com/s/9KEsWqta/edit
| J23103_AB || A9 || Promoter || A || B || Weak Constitutive Promoter || https://benchling.com/s/9KEsWqta/edit
|-
|-
| J23103_EB || A10 || Promoter || EB || Weak Constitutive Promoter || https://benchling.com/s/ynovZBP9/edit
| J23103_EB || A10 || Promoter || E || B || Weak Constitutive Promoter || https://benchling.com/s/ynovZBP9/edit
|-
|-
| J23103_FB || A11 || Promoter || FB || Weak Constitutive Promoter || https://benchling.com/s/oZSwbWfi/edit
| J23103_FB || A11 || Promoter || F || B || Weak Constitutive Promoter || https://benchling.com/s/oZSwbWfi/edit
|-
|-
| J23103_GB || A12 || Promoter || GB || Weak Constitutive Promoter || https://benchling.com/s/4qwyO9ja/edit
| J23103_GB || A12 || Promoter || G || B || Weak Constitutive Promoter || https://benchling.com/s/4qwyO9ja/edit
|-
|-
| J23106_AB || B1 || Promoter || AB || Medium Constitutive Promoter || https://benchling.com/s/riO8LQFW/edit
| J23106_AB || B1 || Promoter || A || B || Medium Constitutive Promoter || https://benchling.com/s/riO8LQFW/edit
|-
|-
| J23106_EB || B2 || Promoter || EB || Medium Constitutive Promoter || https://benchling.com/s/ljtaz48p/edit
| J23106_EB || B2 || Promoter || E || B || Medium Constitutive Promoter || https://benchling.com/s/ljtaz48p/edit
|-
|-
| J23106_FB || B3 || Promoter || FB || Medium Constitutive Promoter || https://benchling.com/s/b8wazW5M/edit
| J23106_FB || B3 || Promoter || F || B || Medium Constitutive Promoter || https://benchling.com/s/b8wazW5M/edit
|-
|-
| J23106_GB || B4 || Promoter || GB || Medium Constitutive Promoter || https://benchling.com/s/UqDZklx7/edit
| J23106_GB || B4 || Promoter || G || B || Medium Constitutive Promoter || https://benchling.com/s/UqDZklx7/edit
|-
|-
| J23107_AB || B5 || Promoter || AB || Medium Constitutive Promoter || https://benchling.com/s/G8ouTflM/edit
| J23107_AB || B5 || Promoter || A || B || Medium Constitutive Promoter || https://benchling.com/s/G8ouTflM/edit
|-
|-
| J23107_EB || B6 || Promoter || EB || Medium Constitutive Promoter || https://benchling.com/s/SGzlA9lZ/edit
| J23107_EB || B6 || Promoter || E || B || Medium Constitutive Promoter || https://benchling.com/s/SGzlA9lZ/edit
|-
|-
| J23107_FB || B7 || Promoter || FB || Medium Constitutive Promoter || https://benchling.com/s/0mSZV0LM/edit
| J23107_FB || B7 || Promoter || F || B || Medium Constitutive Promoter || https://benchling.com/s/0mSZV0LM/edit
|-
|-
| J23107_GB || B8 || Promoter || GB || Medium Constitutive Promoter || https://benchling.com/s/InUihbMF/edit
| J23107_GB || B8 || Promoter || G || B || Medium Constitutive Promoter || https://benchling.com/s/InUihbMF/edit
|-
|-
| J23116_AB || B9 || Promoter || AB || Weak Constitutive Promoter || https://benchling.com/s/1mQPZFtM/edit
| J23116_AB || B9 || Promoter || A || B || Weak Constitutive Promoter || https://benchling.com/s/1mQPZFtM/edit
|-
|-
| J23116_EB || B10 || Promoter || EB || Weak Constitutive Promoter || https://benchling.com/s/xqAO8tJl/edit
| J23116_EB || B10 || Promoter || E || B || Weak Constitutive Promoter || https://benchling.com/s/xqAO8tJl/edit
|-
|-
| J23116_FB || B11 || Promoter || FB || Weak Constitutive Promoter || https://benchling.com/s/o8lvrIdJ/edit
| J23116_FB || B11 || Promoter || F || B || Weak Constitutive Promoter || https://benchling.com/s/o8lvrIdJ/edit
|-
|-
| J23116_GB || B12 || Promoter || GB || Weak Constitutive Promoter || https://benchling.com/s/YYNkGLBV/edit
| J23116_GB || B12 || Promoter || G || B || Weak Constitutive Promoter || https://benchling.com/s/YYNkGLBV/edit
|-
|-
| R0010_AB || C1 || Promoter || AB || pLac LacI Repressible Promoter || https://benchling.com/s/etg7OrN6/edit
| R0010_AB || C1 || Promoter || A || B || pLac LacI Repressible Promoter || https://benchling.com/s/etg7OrN6/edit
|-
|-
| R0010_EB || C2 || Promoter || EB || pLac LacI Repressible Promoter || https://benchling.com/s/FamBps3h/edit
| R0010_EB || C2 || Promoter || E || B || pLac LacI Repressible Promoter || https://benchling.com/s/FamBps3h/edit
|-
|-
| R0010_FB || C3 || Promoter || FB || pLac LacI Repressible Promoter || https://benchling.com/s/oi1T00L0/edit
| R0010_FB || C3 || Promoter || F || B || pLac LacI Repressible Promoter || https://benchling.com/s/oi1T00L0/edit
|-
|-
| R0010_GB || C4 || Promoter || GB || pLac LacI Repressible Promoter || https://benchling.com/s/Yw6bgPjg/edit
| R0010_GB || C4 || Promoter || G || B || pLac LacI Repressible Promoter || https://benchling.com/s/Yw6bgPjg/edit
|-
|-
| R0040_AB || C5 || Promoter || AB || pTet Strong TetR Repressible Promoter || https://benchling.com/s/3RYAjD6u/edit
| R0040_AB || C5 || Promoter || A || B || pTet Strong TetR Repressible Promoter || https://benchling.com/s/3RYAjD6u/edit
|-
|-
| R0040_EB || C6 || Promoter || EB || pTet Strong TetR Repressible Promoter || https://benchling.com/s/SNZHRImR/edit
| R0040_EB || C6 || Promoter || E || B || pTet Strong TetR Repressible Promoter || https://benchling.com/s/SNZHRImR/edit
|-
|-
| R0040_FB || C7 || Promoter || FB || pTet Strong TetR Repressible Promoter || https://benchling.com/s/nyzVqekN/edit
| R0040_FB || C7 || Promoter || F || B || pTet Strong TetR Repressible Promoter || https://benchling.com/s/nyzVqekN/edit
|-
|-
| R0040_GB || C8 || Promoter || GB || pTet Strong TetR Repressible Promoter || https://benchling.com/s/473M59P8/edit
| R0040_GB || C8 || Promoter || G || B || pTet Strong TetR Repressible Promoter || https://benchling.com/s/473M59P8/edit
|-
|-
| R0063_AB || C9 || Promoter || AB || pLux Weak LuxR Repressible Promoter || https://benchling.com/s/EUFuZKHP/edit
| R0063_AB || C9 || Promoter || A || B || pLux Weak LuxR Repressible Promoter || https://benchling.com/s/EUFuZKHP/edit
|-
|-
| R0063_EB || C10 || Promoter || EB || pLux Weak LuxR Repressible Promoter || https://benchling.com/s/PN1VmcWg/edit
| R0063_EB || C10 || Promoter || E || B || pLux Weak LuxR Repressible Promoter || https://benchling.com/s/PN1VmcWg/edit
|-
|-
| R0063_FB || C11 || Promoter || FB || pLux Weak LuxR Repressible Promoter || https://benchling.com/s/xnA98Y5U/edit
| R0063_FB || C11 || Promoter || F || B || pLux Weak LuxR Repressible Promoter || https://benchling.com/s/xnA98Y5U/edit
|-
|-
| R0063_GB || C12 || Promoter || GB || pLux Weak LuxR Repressible Promoter || https://benchling.com/s/jHQHwAaD/edit
| R0063_GB || C12 || Promoter || G || B || pLux Weak LuxR Repressible Promoter || https://benchling.com/s/jHQHwAaD/edit
|-
|-
| I13453_AB || D1 || Promoter || AB || pBAD AraC Inducible Promoter || https://benchling.com/s/HE4joK6k/edit
| I13453_AB || D1 || Promoter || A || B || pBAD AraC Inducible Promoter || https://benchling.com/s/HE4joK6k/edit
|-
|-
| I13453_EB || D2 || Promoter || EB || pBAD AraC Inducible Promoter || https://benchling.com/s/YC8NeyAe/edit
| I13453_EB || D2 || Promoter || E || B || pBAD AraC Inducible Promoter || https://benchling.com/s/YC8NeyAe/edit
|-
|-
| I13453_FB || D3 || Promoter || FB || pBAD AraC Inducible Promoter || https://benchling.com/s/c4zFwYww/edit
| I13453_FB || D3 || Promoter || F || B || pBAD AraC Inducible Promoter || https://benchling.com/s/c4zFwYww/edit
|-
|-
| I13453_GB || D4 || Promoter || GB || pBAD AraC Inducible Promoter || https://benchling.com/s/i9YCgIRi/edit
| I13453_GB || D4 || Promoter || G || B || pBAD AraC Inducible Promoter || https://benchling.com/s/i9YCgIRi/edit
|-
|-
| B0032m_BC || D5 || RBS || BC || Weiss Medium Strength RBS || https://benchling.com/s/jwIsLDg9/edit
| B0032m_BC || D5 || RBS || B || C || Weiss Medium Strength RBS || https://benchling.com/s/jwIsLDg9/edit
|-
|-
| B0033m_BC || D6 || RBS || BC || Weiss Low strength RBS || https://benchling.com/s/FQFaG78R/edit
| B0033m_BC || D6 || RBS || B || C || Weiss Low strength RBS || https://benchling.com/s/FQFaG78R/edit
|-
|-
| B0034m_BC || D7 || RBS || BC || Weiss High Strength RBS || https://benchling.com/s/kBNYDAHo/edit
| B0034m_BC || D7 || RBS || B || C || Weiss High Strength RBS || https://benchling.com/s/kBNYDAHo/edit
|-
|-
| BCD12_BC || D8 || RBS || BC || Medium Strength BCD RBS || https://benchling.com/s/mUcZUqJo/edit
| BCD12_BC || D8 || RBS || B || C || Medium Strength BCD RBS || https://benchling.com/s/mUcZUqJo/edit
|-
|-
| BCD2_BC || D9 || RBS || BC || High Strength BCD RBS || https://benchling.com/s/jM8sZdRg/edit
| BCD2_BC || D9 || RBS || B || C || High Strength BCD RBS || https://benchling.com/s/jM8sZdRg/edit
|-
|-
| BCD8_BC || D10 || RBS || BC || Low Strength BCD RBS || https://benchling.com/s/8UhD5Vaf/edit
| BCD8_BC || D10 || RBS || B || C || Low Strength BCD RBS || https://benchling.com/s/8UhD5Vaf/edit
|-
|-
| C0012m_CD || D11 || CDS || CD || LacI Repressor protein || https://benchling.com/s/1RopAYaI/edit
| C0012m_CD || D11 || CDS || C || D || LacI Repressor protein || https://benchling.com/s/1RopAYaI/edit
|-
|-
| C0040_CD || D12 || CDS || CD || TetR Repressor Protein || https://benchling.com/s/M9uZpTOg/edit
| C0040_CD || D12 || CDS || C || D || TetR Repressor Protein || https://benchling.com/s/M9uZpTOg/edit
|-
|-
| C0062_CD || E1 || CDS || CD || LuxR Repressor/Activator Protein || https://benchling.com/s/FFyxWiwT/edit
| C0062_CD || E1 || CDS || C || D || LuxR Repressor/Activator Protein || https://benchling.com/s/FFyxWiwT/edit
|-
|-
| C0080_CD || E2 || CDS || CD || LuxR Repressor/Activator Protein || https://benchling.com/s/FFyxWiwT/edit
| C0080_CD || E2 || CDS || C || D || LuxR Repressor/Activator Protein || https://benchling.com/s/FFyxWiwT/edit
|-
|-
| cre_CD || E3 || CDS || CD || Cre Recombinase Protein || https://benchling.com/s/CAXPcaMy/edit
| cre_CD || E3 || CDS || C || D || Cre Recombinase Protein || https://benchling.com/s/CAXPcaMy/edit
|-
|-
| E0030_CD || E4 || CDS || CD || Yellow Fluorescent Protein || https://benchling.com/s/cr12BVOE/edit
| E0030_CD || E4 || CDS || C || D || Yellow Fluorescent Protein || https://benchling.com/s/cr12BVOE/edit
|-
|-
| E0040m_CD || E5 || CDS || CD || Green Fluorescent Protein || https://benchling.com/s/cj91KrVH/edit
| E0040m_CD || E5 || CDS || C || D || Green Fluorescent Protein || https://benchling.com/s/cj91KrVH/edit
|-
|-
| E1010m_CD || E6 || CDS || CD || Red Fluorescent Protein || https://benchling.com/s/1AbCv00F/edit
| E1010m_CD || E6 || CDS || C || D || Red Fluorescent Protein || https://benchling.com/s/1AbCv00F/edit
|-
|-
| eBFP2_CD || E7 || CDS || CD || Blue Fluorescent Protein || https://benchling.com/s/bbkctFdr/edit
| eBFP2_CD || E7 || CDS || C || D || Blue Fluorescent Protein || https://benchling.com/s/bbkctFdr/edit
|-
|-
| B0015_DE || E8 || Terminator || DE || Double Terminator || https://benchling.com/s/a1cHND33/edit
| B0015_DE || E8 || Terminator || D || E || Double Terminator || https://benchling.com/s/a1cHND33/edit
|-
|-
| B0015_DF || E9 || Terminator || DF || Double Terminator || https://benchling.com/s/f6RaEG2K/edit
| B0015_DF || E9 || Terminator || D || F || Double Terminator || https://benchling.com/s/f6RaEG2K/edit
|-
|-
| B0015_DG || E10 || Terminator || DG || Double Terminator || https://benchling.com/s/5pFYTkSF/edit
| B0015_DG || E10 || Terminator || D || G || Double Terminator || https://benchling.com/s/5pFYTkSF/edit
|-
|-
| B0015_DH || E11 || Terminator || DH || Double Terminator || https://benchling.com/s/8st8vLlQ/edit
| B0015_DH || E11 || Terminator || D || H || Double Terminator || https://benchling.com/s/8st8vLlQ/edit
|-
|-
| DVA_AB || E12 || Destination || AB || Moclo Part Destination Vector (stage 1), Promoter || https://benchling.com/s/slHkWK6p/edit
| DVA_AB || E12 || Destination || A || B || Moclo Part Destination Vector (stage 1), Promoter || https://benchling.com/s/slHkWK6p/edit
|-
|-
| DVA_AE || F1 || Destination || AE || Moclo Device Destination Vector (stage 3) || https://benchling.com/s/JbqtviMA/edit
| DVA_AE || F1 || Destination || A || E || Moclo Device Destination Vector (stage 3) || https://benchling.com/s/JbqtviMA/edit
|-
|-
| DVA_AF || F2 || Destination || AF || Moclo Device Destination Vector (stage 3) || https://benchling.com/s/NviiNLIp/edit
| DVA_AF || F2 || Destination || A || F || Moclo Device Destination Vector (stage 3) || https://benchling.com/s/NviiNLIp/edit
|-
|-
| DVA_AG || F3 || Destination || AG || Moclo Device Destination Vector (stage 3) || https://benchling.com/s/pPe92CO5/edit
| DVA_AG || F3 || Destination || A || G || Moclo Device Destination Vector (stage 3) || https://benchling.com/s/pPe92CO5/edit
|-
|-
| DVA_AH || F4 || Destination || AH || Moclo Device Destination Vector (stage 3) || https://benchling.com/s/iaYDvlV2/edit
| DVA_AH || F4 || Destination || A || H || Moclo Device Destination Vector (stage 3) || https://benchling.com/s/iaYDvlV2/edit
|-
|-
| DVA_BC || F5 || Destination || BC || Moclo Part Destination Vector (stage 1), UTR || https://benchling.com/s/o7TOrROp/edit
| DVA_BC || F5 || Destination || B || C || Moclo Part Destination Vector (stage 1), UTR || https://benchling.com/s/o7TOrROp/edit
|-
|-
| DVA_CD || F6 || Destination || CD || Moclo Part Destination Vector (stage 1), CDS || https://benchling.com/s/xgdB8Znv/edit
| DVA_CD || F6 || Destination || C || D || Moclo Part Destination Vector (stage 1), CDS || https://benchling.com/s/xgdB8Znv/edit
|-
|-
| DVA_DE || F7 || Destination || DE || Moclo Part Destination Vector (stage 1), Terminator || https://benchling.com/s/mtpMynxT/edit
| DVA_DE || F7 || Destination || D || E || Moclo Part Destination Vector (stage 1), Terminator || https://benchling.com/s/mtpMynxT/edit
|-
|-
| DVA_DF || F8 || Destination || DF || Moclo Part Destination Vector (stage 1), Terminator || https://benchling.com/s/53Whxb2s/edit
| DVA_DF || F8 || Destination || D || F || Moclo Part Destination Vector (stage 1), Terminator || https://benchling.com/s/53Whxb2s/edit
|-
|-
| DVA_DG || F9 || Destination || DG || Moclo Part Destination Vector (stage 1), Terminator || https://benchling.com/s/bJdQOwZP/edit
| DVA_DG || F9 || Destination || D || G || Moclo Part Destination Vector (stage 1), Terminator || https://benchling.com/s/bJdQOwZP/edit
|-
|-
| DVA_DH || F10 || Destination || DH || Moclo Part Destination Vector (stage 1), Terminator || https://benchling.com/s/sGGQDAg6/edit
| DVA_DH || F10 || Destination || D || H || Moclo Part Destination Vector (stage 1), Terminator || https://benchling.com/s/sGGQDAg6/edit
|-
|-
| DVA_EB || F11 || Destination || EB || Moclo Part Destination Vector (stage 1), Promoter || https://benchling.com/s/i5JEIhUg/edit
| DVA_EB || F11 || Destination || E || B || Moclo Part Destination Vector (stage 1), Promoter || https://benchling.com/s/i5JEIhUg/edit
|-
|-
| DVA_EF || F12 || Destination || EF || Moclo Device Destination Vector (stage 3) || https://benchling.com/s/OlSBaZCj/edit
| DVA_EF || F12 || Destination || E || F || Moclo Device Destination Vector (stage 3) || https://benchling.com/s/OlSBaZCj/edit
|-
|-
| DVA_EG || G1 || Destination || EG || Moclo Device Destination Vector (stage 3) || https://benchling.com/s/xGeYtbZx/edit
| DVA_EG || G1 || Destination || E || EG || Moclo Device Destination Vector (stage 3) || https://benchling.com/s/xGeYtbZx/edit
|-
|-
| DVA_EH || G2 || Destination || EH || Moclo Device Destination Vector (stage 3) || https://benchling.com/s/fnTqha2S/edit
| DVA_EH || G2 || Destination || E || EH || Moclo Device Destination Vector (stage 3) || https://benchling.com/s/fnTqha2S/edit
|-
|-
| DVA_FB || G3 || Destination || FB || Moclo Part Destination Vector (stage 1), Promoter || https://benchling.com/s/3CuYwFwU/edit
| DVA_FB || G3 || Destination || F || FB || Moclo Part Destination Vector (stage 1), Promoter || https://benchling.com/s/3CuYwFwU/edit
|-
|-
| DVA_GB || G4 || Destination || GB || Moclo Part Destination Vector (stage 1), Promoter || https://benchling.com/s/UvuVapbV/edit
| DVA_GB || G4 || Destination || G || GB || Moclo Part Destination Vector (stage 1), Promoter || https://benchling.com/s/UvuVapbV/edit
|-
|-
| DVA || G5 || Vector || - || Vector for new Stage 1 or 3 vector || https://benchling.com/s/6hijqfvT/edit
| DVA || G5 || Vector || - || - || Vector for new Stage 1 or 3 vector || https://benchling.com/s/6hijqfvT/edit
|-
|-
| pJ02B2Gm_AE || G6 || Device || AE || High GFP Expression Device, in Part Vector || https://benchling.com/s/mgYDcL4z/edit
| pJ02B2Gm_AE || G6 || Device || A || E || High GFP Expression Device, in Part Vector || https://benchling.com/s/mgYDcL4z/edit
|-
|-
| pJ02B2Gm_EF || G7 || Device || EF || High GFP Expression Device, in Part Vector || https://benchling.com/s/3WQbdlzU/edit
| pJ02B2Gm_EF || G7 || Device || E || F || High GFP Expression Device, in Part Vector || https://benchling.com/s/3WQbdlzU/edit
|-
|-
| pJ02B2Rm_AE || G8 || Device || AE || High RFP Expression Device, in Part Vector || https://benchling.com/s/Kb4AAbox/edit
| pJ02B2Rm_AE || G8 || Device || A || E || High RFP Expression Device, in Part Vector || https://benchling.com/s/Kb4AAbox/edit
|-
|-
| pJ02B2Rm_EF || G9 || Device || EF || High RFP Expression Device, in Part Vector || https://benchling.com/s/FOM8uxtS/edit
| pJ02B2Rm_EF || G9 || Device || E || F || High RFP Expression Device, in Part Vector || https://benchling.com/s/FOM8uxtS/edit
|-
|-
| pJ02B2Gm:Rm_AF || G10 || Device || AF || High GFP and High RFP expression Device, in Part Vector || https://benchling.com/s/D75ziv6K/edit
| pJ02B2Gm:Rm_AF || G10 || Device || A || F || High GFP and High RFP expression Device, in Part Vector || https://benchling.com/s/D75ziv6K/edit
|-
|-
| pJ02B2Rm:Gm_AF || G11 || Device || AF || High RFP and High GFP Expression Device, in Part Vector || https://benchling.com/s/OOkaVw1a/edit
| pJ02B2Rm:Gm_AF || G11 || Device || A || F || High RFP and High GFP Expression Device, in Part Vector || https://benchling.com/s/OOkaVw1a/edit
|-
|-
| pJ02B2Gm_AE || G12 || Device || AE || High GFP Expression Device, in Stage 2 Vector || https://benchling.com/s/ShVJxDdF/edit
| pJ02B2Gm_AE || G12 || Device || A || E || High GFP Expression Device, in Stage 2 Vector || https://benchling.com/s/ShVJxDdF/edit
|-
|-
| pJ02B2Gm_EF || H1 || Device || EF || High GFP Expression Device, in Stage 2 Vector || https://benchling.com/s/uUTmefO7/edit
| pJ02B2Gm_EF || H1 || Device || E || F || High GFP Expression Device, in Stage 2 Vector || https://benchling.com/s/uUTmefO7/edit
|-
|-
| pJ02B2Rm_AE || H2 || Device || AE || High RFP Expression Device, in Stage 2 Vector || https://benchling.com/s/syrZcMB4/edit
| pJ02B2Rm_AE || H2 || Device || A || E || High RFP Expression Device, in Stage 2 Vector || https://benchling.com/s/syrZcMB4/edit
|-
|-
| pJ02B2Rm_EF || H3 || Device || EF || High RFP Expression Device, in Stage 2 Vector || https://benchling.com/s/nNICVGuk/edit
| pJ02B2Rm_EF || H3 || Device || E || F || High RFP Expression Device, in Stage 2 Vector || https://benchling.com/s/nNICVGuk/edit
|-
|-
| DVK_AE || H4 || Destination || AE || Moclo Device Destination Vector (stage 2) || https://benchling.com/s/sueyphOw/edit
| DVK_AE || H4 || Destination || A || E || Moclo Device Destination Vector (stage 2) || https://benchling.com/s/sueyphOw/edit
|-
|-
| DVK_AF || H5 || Destination || AF || Moclo Device Destination Vector (stage 2) || https://benchling.com/s/1i0mX41y/edit
| DVK_AF || H5 || Destination || A || F || Moclo Device Destination Vector (stage 2) || https://benchling.com/s/1i0mX41y/edit
|-
|-
| DVK_EF || H6 || Destination || EF || Moclo Device Destination Vector (stage 2) || https://benchling.com/s/7FuJO5tD/edit
| DVK_EF || H6 || Destination || E || F || Moclo Device Destination Vector (stage 2) || https://benchling.com/s/7FuJO5tD/edit
|-
|-
| DVK_FG || H7 || Destination || FG || Moclo Device Destination Vector (stage 2) || https://benchling.com/s/Cz5hQ9wM/edit
| DVK_FG || H7 || Destination || F || G || Moclo Device Destination Vector (stage 2) || https://benchling.com/s/Cz5hQ9wM/edit
|-
|-
| DVK_GH || H8 || Destination || GH || Moclo Device Destination Vector (stage 2) || https://benchling.com/s/MFw9pNDU/edit
| DVK_GH || H8 || Destination || G || H || Moclo Device Destination Vector (stage 2) || https://benchling.com/s/MFw9pNDU/edit
|-
|-
| DVK || H9 || Vector || - || Vector for new Stage 2 vector || https://benchling.com/s/olS42cDR/edit
| DVK || H9 || Vector || - || - || Vector for new Stage 2 vector || https://benchling.com/s/olS42cDR/edit
|}
|}

Revision as of 02:48, 22 June 2016

CIDAR.png

Moclo is a type of cloning strategy that attempts to standardize the assembly of multiple DNA parts into finished constructs or 'devices'. The basic cloning method relies on the fact that typeIIs restriction enzymes cut outside their sequence of recognition. Unlike traditional restriction cloning, this means there is no chance that ligated pieces can be cut again by the same enzyme. This also gives you complete freedom in choosing the four base-pair sticky end, and allows multiple specific ligations to be accomplished in a single pot, with only one restriction enzyme.

In order to take full advantage of the modularity and ease of use, one must spend significant time "domesticating" DNA parts by removing extra restriction sites and placing them into appropriate vectors which contain the typeIIs restriction sites in the right orientation. This work has been done by many labs (with equally many sticky end overhang standards).

One example of such a standard is the CIDAR library from Doug Densmore's lab

CIDAR overhangs

A Promoter B RBS C CDS D Term E F G H
GGAG TACT AATG AGGT GCTT CGCT TGCC ACTA

CIDAR parts list

Name Well Type Left Right Description Link
J23100_AB A1 Promoter A B Weak Constitutive Promoter https://benchling.com/s/gS6lXC3X/edit
J23100_EB A2 Promoter E B Weak Constitutive Promoter https://benchling.com/s/aq8Gxp2O/edit
J23100_FB A3 Promoter F B Weak Constitutive Promoter https://benchling.com/s/rTzq9ToN/edit
J23100_GB A4 Promoter G B Weak Constitutive Promoter https://benchling.com/s/5yn5Zy6g/edit
J23102_AB A5 Promoter A B Strong Constitutive Promoter https://benchling.com/s/aQOLd1xr/edit
J23102_EB A6 Promoter E B Strong Constitutive Promoter https://benchling.com/s/zOCQC6dM/edit
J23102_FB A7 Promoter F B Strong Constitutive Promoter https://benchling.com/s/1pfiY4NG/edit
J23102_GB A8 Promoter G B Strong Constitutive Promoter https://benchling.com/s/LmBWThyt/edit
J23103_AB A9 Promoter A B Weak Constitutive Promoter https://benchling.com/s/9KEsWqta/edit
J23103_EB A10 Promoter E B Weak Constitutive Promoter https://benchling.com/s/ynovZBP9/edit
J23103_FB A11 Promoter F B Weak Constitutive Promoter https://benchling.com/s/oZSwbWfi/edit
J23103_GB A12 Promoter G B Weak Constitutive Promoter https://benchling.com/s/4qwyO9ja/edit
J23106_AB B1 Promoter A B Medium Constitutive Promoter https://benchling.com/s/riO8LQFW/edit
J23106_EB B2 Promoter E B Medium Constitutive Promoter https://benchling.com/s/ljtaz48p/edit
J23106_FB B3 Promoter F B Medium Constitutive Promoter https://benchling.com/s/b8wazW5M/edit
J23106_GB B4 Promoter G B Medium Constitutive Promoter https://benchling.com/s/UqDZklx7/edit
J23107_AB B5 Promoter A B Medium Constitutive Promoter https://benchling.com/s/G8ouTflM/edit
J23107_EB B6 Promoter E B Medium Constitutive Promoter https://benchling.com/s/SGzlA9lZ/edit
J23107_FB B7 Promoter F B Medium Constitutive Promoter https://benchling.com/s/0mSZV0LM/edit
J23107_GB B8 Promoter G B Medium Constitutive Promoter https://benchling.com/s/InUihbMF/edit
J23116_AB B9 Promoter A B Weak Constitutive Promoter https://benchling.com/s/1mQPZFtM/edit
J23116_EB B10 Promoter E B Weak Constitutive Promoter https://benchling.com/s/xqAO8tJl/edit
J23116_FB B11 Promoter F B Weak Constitutive Promoter https://benchling.com/s/o8lvrIdJ/edit
J23116_GB B12 Promoter G B Weak Constitutive Promoter https://benchling.com/s/YYNkGLBV/edit
R0010_AB C1 Promoter A B pLac LacI Repressible Promoter https://benchling.com/s/etg7OrN6/edit
R0010_EB C2 Promoter E B pLac LacI Repressible Promoter https://benchling.com/s/FamBps3h/edit
R0010_FB C3 Promoter F B pLac LacI Repressible Promoter https://benchling.com/s/oi1T00L0/edit
R0010_GB C4 Promoter G B pLac LacI Repressible Promoter https://benchling.com/s/Yw6bgPjg/edit
R0040_AB C5 Promoter A B pTet Strong TetR Repressible Promoter https://benchling.com/s/3RYAjD6u/edit
R0040_EB C6 Promoter E B pTet Strong TetR Repressible Promoter https://benchling.com/s/SNZHRImR/edit
R0040_FB C7 Promoter F B pTet Strong TetR Repressible Promoter https://benchling.com/s/nyzVqekN/edit
R0040_GB C8 Promoter G B pTet Strong TetR Repressible Promoter https://benchling.com/s/473M59P8/edit
R0063_AB C9 Promoter A B pLux Weak LuxR Repressible Promoter https://benchling.com/s/EUFuZKHP/edit
R0063_EB C10 Promoter E B pLux Weak LuxR Repressible Promoter https://benchling.com/s/PN1VmcWg/edit
R0063_FB C11 Promoter F B pLux Weak LuxR Repressible Promoter https://benchling.com/s/xnA98Y5U/edit
R0063_GB C12 Promoter G B pLux Weak LuxR Repressible Promoter https://benchling.com/s/jHQHwAaD/edit
I13453_AB D1 Promoter A B pBAD AraC Inducible Promoter https://benchling.com/s/HE4joK6k/edit
I13453_EB D2 Promoter E B pBAD AraC Inducible Promoter https://benchling.com/s/YC8NeyAe/edit
I13453_FB D3 Promoter F B pBAD AraC Inducible Promoter https://benchling.com/s/c4zFwYww/edit
I13453_GB D4 Promoter G B pBAD AraC Inducible Promoter https://benchling.com/s/i9YCgIRi/edit
B0032m_BC D5 RBS B C Weiss Medium Strength RBS https://benchling.com/s/jwIsLDg9/edit
B0033m_BC D6 RBS B C Weiss Low strength RBS https://benchling.com/s/FQFaG78R/edit
B0034m_BC D7 RBS B C Weiss High Strength RBS https://benchling.com/s/kBNYDAHo/edit
BCD12_BC D8 RBS B C Medium Strength BCD RBS https://benchling.com/s/mUcZUqJo/edit
BCD2_BC D9 RBS B C High Strength BCD RBS https://benchling.com/s/jM8sZdRg/edit
BCD8_BC D10 RBS B C Low Strength BCD RBS https://benchling.com/s/8UhD5Vaf/edit
C0012m_CD D11 CDS C D LacI Repressor protein https://benchling.com/s/1RopAYaI/edit
C0040_CD D12 CDS C D TetR Repressor Protein https://benchling.com/s/M9uZpTOg/edit
C0062_CD E1 CDS C D LuxR Repressor/Activator Protein https://benchling.com/s/FFyxWiwT/edit
C0080_CD E2 CDS C D LuxR Repressor/Activator Protein https://benchling.com/s/FFyxWiwT/edit
cre_CD E3 CDS C D Cre Recombinase Protein https://benchling.com/s/CAXPcaMy/edit
E0030_CD E4 CDS C D Yellow Fluorescent Protein https://benchling.com/s/cr12BVOE/edit
E0040m_CD E5 CDS C D Green Fluorescent Protein https://benchling.com/s/cj91KrVH/edit
E1010m_CD E6 CDS C D Red Fluorescent Protein https://benchling.com/s/1AbCv00F/edit
eBFP2_CD E7 CDS C D Blue Fluorescent Protein https://benchling.com/s/bbkctFdr/edit
B0015_DE E8 Terminator D E Double Terminator https://benchling.com/s/a1cHND33/edit
B0015_DF E9 Terminator D F Double Terminator https://benchling.com/s/f6RaEG2K/edit
B0015_DG E10 Terminator D G Double Terminator https://benchling.com/s/5pFYTkSF/edit
B0015_DH E11 Terminator D H Double Terminator https://benchling.com/s/8st8vLlQ/edit
DVA_AB E12 Destination A B Moclo Part Destination Vector (stage 1), Promoter https://benchling.com/s/slHkWK6p/edit
DVA_AE F1 Destination A E Moclo Device Destination Vector (stage 3) https://benchling.com/s/JbqtviMA/edit
DVA_AF F2 Destination A F Moclo Device Destination Vector (stage 3) https://benchling.com/s/NviiNLIp/edit
DVA_AG F3 Destination A G Moclo Device Destination Vector (stage 3) https://benchling.com/s/pPe92CO5/edit
DVA_AH F4 Destination A H Moclo Device Destination Vector (stage 3) https://benchling.com/s/iaYDvlV2/edit
DVA_BC F5 Destination B C Moclo Part Destination Vector (stage 1), UTR https://benchling.com/s/o7TOrROp/edit
DVA_CD F6 Destination C D Moclo Part Destination Vector (stage 1), CDS https://benchling.com/s/xgdB8Znv/edit
DVA_DE F7 Destination D E Moclo Part Destination Vector (stage 1), Terminator https://benchling.com/s/mtpMynxT/edit
DVA_DF F8 Destination D F Moclo Part Destination Vector (stage 1), Terminator https://benchling.com/s/53Whxb2s/edit
DVA_DG F9 Destination D G Moclo Part Destination Vector (stage 1), Terminator https://benchling.com/s/bJdQOwZP/edit
DVA_DH F10 Destination D H Moclo Part Destination Vector (stage 1), Terminator https://benchling.com/s/sGGQDAg6/edit
DVA_EB F11 Destination E B Moclo Part Destination Vector (stage 1), Promoter https://benchling.com/s/i5JEIhUg/edit
DVA_EF F12 Destination E F Moclo Device Destination Vector (stage 3) https://benchling.com/s/OlSBaZCj/edit
DVA_EG G1 Destination E EG Moclo Device Destination Vector (stage 3) https://benchling.com/s/xGeYtbZx/edit
DVA_EH G2 Destination E EH Moclo Device Destination Vector (stage 3) https://benchling.com/s/fnTqha2S/edit
DVA_FB G3 Destination F FB Moclo Part Destination Vector (stage 1), Promoter https://benchling.com/s/3CuYwFwU/edit
DVA_GB G4 Destination G GB Moclo Part Destination Vector (stage 1), Promoter https://benchling.com/s/UvuVapbV/edit
DVA G5 Vector - - Vector for new Stage 1 or 3 vector https://benchling.com/s/6hijqfvT/edit
pJ02B2Gm_AE G6 Device A E High GFP Expression Device, in Part Vector https://benchling.com/s/mgYDcL4z/edit
pJ02B2Gm_EF G7 Device E F High GFP Expression Device, in Part Vector https://benchling.com/s/3WQbdlzU/edit
pJ02B2Rm_AE G8 Device A E High RFP Expression Device, in Part Vector https://benchling.com/s/Kb4AAbox/edit
pJ02B2Rm_EF G9 Device E F High RFP Expression Device, in Part Vector https://benchling.com/s/FOM8uxtS/edit
pJ02B2Gm:Rm_AF G10 Device A F High GFP and High RFP expression Device, in Part Vector https://benchling.com/s/D75ziv6K/edit
pJ02B2Rm:Gm_AF G11 Device A F High RFP and High GFP Expression Device, in Part Vector https://benchling.com/s/OOkaVw1a/edit
pJ02B2Gm_AE G12 Device A E High GFP Expression Device, in Stage 2 Vector https://benchling.com/s/ShVJxDdF/edit
pJ02B2Gm_EF H1 Device E F High GFP Expression Device, in Stage 2 Vector https://benchling.com/s/uUTmefO7/edit
pJ02B2Rm_AE H2 Device A E High RFP Expression Device, in Stage 2 Vector https://benchling.com/s/syrZcMB4/edit
pJ02B2Rm_EF H3 Device E F High RFP Expression Device, in Stage 2 Vector https://benchling.com/s/nNICVGuk/edit
DVK_AE H4 Destination A E Moclo Device Destination Vector (stage 2) https://benchling.com/s/sueyphOw/edit
DVK_AF H5 Destination A F Moclo Device Destination Vector (stage 2) https://benchling.com/s/1i0mX41y/edit
DVK_EF H6 Destination E F Moclo Device Destination Vector (stage 2) https://benchling.com/s/7FuJO5tD/edit
DVK_FG H7 Destination F G Moclo Device Destination Vector (stage 2) https://benchling.com/s/Cz5hQ9wM/edit
DVK_GH H8 Destination G H Moclo Device Destination Vector (stage 2) https://benchling.com/s/MFw9pNDU/edit
DVK H9 Vector - - Vector for new Stage 2 vector https://benchling.com/s/olS42cDR/edit
Retrieved from "https://murray.cds.caltech.edu/index.php?title=Moclo&oldid=20731"