Moclo - Revision history

Ashur: Replaced content with "See here https://www.cds.caltech.edu/biocircuits/index.php/Moclo"

2016-06-29T19:14:40Z

Replaced content with "See here https://www.cds.caltech.edu/biocircuits/index.php/Moclo"

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Ashur: /* CIDAR parts list */

2016-06-22T02:48:39Z

CIDAR parts list

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Ashur at 02:42, 22 June 2016

2016-06-22T02:42:54Z

← Older revision		Revision as of 02:42, 22 June 2016
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	One example of such a standard is the CIDAR library from [http://pubs.acs.org/doi/pdf/10.1021/acssynbio.5b00124 Doug Densmore's lab]		One example of such a standard is the CIDAR library from [http://pubs.acs.org/doi/pdf/10.1021/acssynbio.5b00124 Doug Densmore's lab]
			=CIDAR overhangs=
			{\| class="wikitable"
			! A !!Promoter !! B !! RBS !! C !! CDS !! D !! Term !! E !! F !! G !! H
			\|-
			\| GGAG \|\|\|\| TACT \|\|\|\| AATG \|\|\|\| AGGT \|\|\|\| GCTT \|\| CGCT \|\| TGCC \|\| ACTA

			\|}
	=CIDAR parts list=		=CIDAR parts list=

Ashur at 02:29, 22 June 2016

2016-06-22T02:29:03Z

← Older revision		Revision as of 02:29, 22 June 2016
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	[[File:CIDAR.png\|right\|450px]]		[[File:CIDAR.png\|right\|450px]]
	Moclo is a type of cloning strategy that attempts to standardize the assembly of multiple DNA parts into finished constructs or 'devices'. The basic cloning method relies on the fact that typeIIs restriction enzymes cut outside their sequence of recognition. Unlike traditional restriction cloning, this means there is no chance that ligated pieces can be cut again by the same enzyme. This also gives you complete freedom in choosing the four base-pair sticky end, and allows multiple specific ligations to be accomplished with only one restriction enzyme.		Moclo is a type of cloning strategy that attempts to standardize the assembly of multiple DNA parts into finished constructs or 'devices'. The basic cloning method relies on the fact that typeIIs restriction enzymes cut outside their sequence of recognition. Unlike traditional restriction cloning, this means there is no chance that ligated pieces can be cut again by the same enzyme. This also gives you complete freedom in choosing the four base-pair sticky end, and allows multiple specific ligations to be accomplished in a single pot, with only one restriction enzyme.

	In order to take full advantage of the modularity and ease of use, one must spend significant time "domesticating" DNA parts by removing extra restriction sites and placing them into appropriate vectors which contain the typeIIs restriction sites in the right orientation. This work has been done by many labs (with equally many sticky end overhang standards).		In order to take full advantage of the modularity and ease of use, one must spend significant time "domesticating" DNA parts by removing extra restriction sites and placing them into appropriate vectors which contain the typeIIs restriction sites in the right orientation. This work has been done by many labs (with equally many sticky end overhang standards).

Ashur at 02:27, 22 June 2016

2016-06-22T02:27:47Z

← Older revision		Revision as of 02:27, 22 June 2016
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			[[File:CIDAR.png\|right\|450px]]
	Moclo is a type of cloning strategy that attempts to standardize the assembly of multiple DNA parts into finished constructs or 'devices'. The basic cloning method relies on the fact that typeIIs restriction enzymes cut outside their sequence of recognition. Unlike traditional restriction cloning, this means there is no chance that ligated pieces can be cut again by the same enzyme. This also gives you complete freedom in choosing the four base-pair sticky end, and allows multiple specific ligations to be accomplished with only one restriction enzyme.		Moclo is a type of cloning strategy that attempts to standardize the assembly of multiple DNA parts into finished constructs or 'devices'. The basic cloning method relies on the fact that typeIIs restriction enzymes cut outside their sequence of recognition. Unlike traditional restriction cloning, this means there is no chance that ligated pieces can be cut again by the same enzyme. This also gives you complete freedom in choosing the four base-pair sticky end, and allows multiple specific ligations to be accomplished with only one restriction enzyme.

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	One example of such a standard is the CIDAR library from [http://pubs.acs.org/doi/pdf/10.1021/acssynbio.5b00124 Doug Densmore's lab]		One example of such a standard is the CIDAR library from [http://pubs.acs.org/doi/pdf/10.1021/acssynbio.5b00124 Doug Densmore's lab]

	=CIDAR=		=CIDAR parts list=
	~~[[File:CIDAR.png\|right\|500px]]~~
	{\| class="wikitable sortable"		{\| class="wikitable sortable"
	! Name !! Well !! Type !! Overhangs !! Description !! Link		! Name !! Well !! Type !! Overhangs !! Description !! Link

Ashur at 02:25, 22 June 2016

2016-06-22T02:25:27Z

← Older revision		Revision as of 02:25, 22 June 2016
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	In order to take full advantage of the modularity and ease of use, one must spend significant time "domesticating" DNA parts by removing extra restriction sites and placing them into appropriate vectors which contain the typeIIs restriction sites in the right orientation. This work has been done by many labs (with equally many sticky end overhang standards).		In order to take full advantage of the modularity and ease of use, one must spend significant time "domesticating" DNA parts by removing extra restriction sites and placing them into appropriate vectors which contain the typeIIs restriction sites in the right orientation. This work has been done by many labs (with equally many sticky end overhang standards).

	One example of such a standard is the CIDAR library from Doug Densmore's lab		One example of such a standard is the CIDAR library from [http://pubs.acs.org/doi/pdf/10.1021/acssynbio.5b00124 Doug Densmore's lab]

	=CIDAR=		=CIDAR=

Ashur: /* CIDAR */

2016-06-22T02:24:01Z

CIDAR

← Older revision		Revision as of 02:24, 22 June 2016
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	=CIDAR=		=CIDAR=
	[[File:CIDAR.png]]		[[File:CIDAR.png\|right\|500px]]
	{\| class="wikitable sortable"		{\| class="wikitable sortable"
	! Name !! Well !! Type !! Overhangs !! Description !! Link		! Name !! Well !! Type !! Overhangs !! Description !! Link

Ashur at 02:21, 22 June 2016

2016-06-22T02:21:09Z

← Older revision		Revision as of 02:21, 22 June 2016
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	=CIDAR=		=CIDAR=
			[[File:CIDAR.png]]
	{\| class="wikitable sortable"		{\| class="wikitable sortable"
	! Name !! Well !! Type !! Overhangs !! Description !! Link		! Name !! Well !! Type !! Overhangs !! Description !! Link

Ashur at 02:18, 22 June 2016

2016-06-22T02:18:26Z

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Ashur at 01:32, 22 June 2016

2016-06-22T01:32:22Z

← Older revision		Revision as of 01:32, 22 June 2016
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	In order to take full advantage of the modularity and ease of use, one must spend significant time "domesticating" DNA parts by removing extra restriction sites and placing them into appropriate vectors which contain the typeIIs restriction sites in the right orientation. This work has been done by many labs (with equally many sticky end overhang standards).		In order to take full advantage of the modularity and ease of use, one must spend significant time "domesticating" DNA parts by removing extra restriction sites and placing them into appropriate vectors which contain the typeIIs restriction sites in the right orientation. This work has been done by many labs (with equally many sticky end overhang standards).
	~~===~~
	One example of		One example of such a standard is the CIDAR library from Doug Densmore's lab
			CIDAR==

			== library contents ==