Design of a Toolbox of RNA Thermometers - Revision history

Murray at 21:02, 11 June 2016

2016-06-11T21:02:00Z

← Older revision		Revision as of 21:02, 11 June 2016
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	\| year = 2015		\| year = 2015
	\| type = Conference Paper		\| type = Conference Paper
	\| funding = ~~MPP~~		\| funding = MPP2
	\| url = http://biorxiv.org/content/early/2015/03/30/017269		\| url = http://biorxiv.org/content/early/2015/03/30/017269
	\| abstract =		\| abstract =

Murray at 16:34, 15 May 2016

2016-05-15T16:34:39Z

← Older revision		Revision as of 16:34, 15 May 2016
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	\| url = http://biorxiv.org/content/early/2015/03/30/017269		\| url = http://biorxiv.org/content/early/2015/03/30/017269
	\| abstract =		\| abstract =
	RNA thermometers mediate responses to temperature changes in various natural circuits, and have been developed in a synthetic context as well. However, a toolbox of RNA thermometers with diâµerent sensitivities to temperature is lacking. Here, we address this issue using a combination of computational and experimental methodologies. We analysed a set of available synthetic RNA thermometers through a quantification of their activity as a function of temperatures in a cell- free expression molecular breadboard system as well as through computation of their melt profiles. Based on this, we computed melt profiles of a library of RNA thermometers and found that the library contained RNA thermometers with a range of sensitivities and thresholds in their response to temperature. We constructed this library and found, through preliminary measurements, a wide range of responses to temperature, which in some cases matched the computational predictions. The constructed library represents a toolbox of RNA thermometers with different sensitivities and is foun- dational work towards synthetic biology applications such as ~~e�cient~~ control of large volume chemical reactors, precise spatiotemporal control of gene expression as well as tools to engineer robustness to temperature in biomolecular circuits.		RNA thermometers mediate responses to temperature changes in various natural circuits, and have been developed in a synthetic context as well. However, a toolbox of RNA thermometers with diâµerent sensitivities to temperature is lacking. Here, we address this issue using a combination of computational and experimental methodologies. We analysed a set of available synthetic RNA thermometers through a quantification of their activity as a function of temperatures in a cell- free expression molecular breadboard system as well as through computation of their melt profiles. Based on this, we computed melt profiles of a library of RNA thermometers and found that the library contained RNA thermometers with a range of sensitivities and thresholds in their response to temperature. We constructed this library and found, through preliminary measurements, a wide range of responses to temperature, which in some cases matched the computational predictions. The constructed library represents a toolbox of RNA thermometers with different sensitivities and is foun- dational work towards synthetic biology applications such as efficient control of large volume chemical reactors, precise spatiotemporal control of gene expression as well as tools to engineer robustness to temperature in biomolecular circuits.

	\| flags =		\| flags =

Murray: htdb2wiki: creating page for 2015b_sssm15-seed.html

2016-05-15T05:39:26Z

htdb2wiki: creating page for 2015b_sssm15-seed.html

New page

{{HTDB paper
| authors = Rohit Satija, Shaunak Sen, Dan Siegal-Gaskins, Richard M. Murray
| title = Design of a Toolbox of RNA Thermometers
| source = Presented at Synthetic Biology: Engineering, Evolution and Design (SEED), 10-13 June 2015
| year = 2015
| type = Conference Paper
| funding = MPP
| url = http://biorxiv.org/content/early/2015/03/30/017269
| abstract =
RNA thermometers mediate responses to temperature changes in various natural circuits, and have been developed in a synthetic context as well. However, a toolbox of RNA thermometers with diâµerent sensitivities to temperature is lacking. Here, we address this issue using a combination of computational and experimental methodologies. We analysed a set of available synthetic RNA thermometers through a quantification of their activity as a function of temperatures in a cell- free expression molecular breadboard system as well as through computation of their melt profiles. Based on this, we computed melt profiles of a library of RNA thermometers and found that the library contained RNA thermometers with a range of sensitivities and thresholds in their response to temperature. We constructed this library and found, through preliminary measurements, a wide range of responses to temperature, which in some cases matched the computational predictions. The constructed library represents a toolbox of RNA thermometers with different sensitivities and is foun- dational work towards synthetic biology applications such as e�cient control of large volume chemical reactors, precise spatiotemporal control of gene expression as well as tools to engineer robustness to temperature in biomolecular circuits.

| flags =
| filetype = link
| tag = sssm15-seed
| id = 2015b
}}