BFS GOTChAs, April 2010 - Revision history

Murray at 17:39, 1 May 2010

2010-05-01T17:39:22Z

← Older revision		Revision as of 17:39, 1 May 2010
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	=== State Transition Modeling in Chemical Reaction Networks (JTM and ES) ===		=== State Transition Modeling in Chemical Reaction Networks (JTM and ES) ===
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2010-05-01T17:38:58Z

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 This page contains some [[GOTChA Chart|GOTChA charts]] for possible rotation projects in the lab this summer. __NOTOC__
 == Device Projects ==
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 ==== Approach ====
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2010-04-30T11:18:11Z

Device Projects

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 ** Could some signaling peptide be integrated into the protein such that it's released upon degradation via a non-processive protease?
 ** For a 1:1 stoichiometry, one could imagine simply fusing the two proteins and then also expressing a protease to separate them after translation
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 ==== Approach ====

2010-04-30T10:54:56Z

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 ** Could some signaling peptide be integrated into the protein such that it's released upon degradation via a non-processive protease?
 ** For a 1:1 stoichiometry, one could imagine simply fusing the two proteins and then also expressing a protease to separate them after translation
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 ==== Approach ====

2010-04-30T10:31:13Z

Device Projects

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 ** The simplest approach would be to use a reversible recombinase (like fin and hin [http://jb.asm.org/cgi/content/full/188/3/950]) under a cell cycle dependent promoter
 ** If we see too many double inversions need to device a more complex mechanism using a combination of recombinases
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2010-04-30T10:23:12Z

Robustness Projects

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 * Design theoretical circuit, then use canonical examples of reactions
 * Try the a fusion protein with the circuit that is tightly regulated in paper as a crutch
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2010-04-30T06:53:17Z

Robustness Projects

← Older revision		Revision as of 06:53, 30 April 2010
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	=== Consistent Gene Expression Level (ES) ===		=== Consistent Gene Expression Level (ES and JTM) ===
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	==== Approach ====		==== Approach ====
	* Incorporate feedback into current inducer systems, possibly tie into a gene that is known to be tightly controlled		* Incorporate feedback into current inducer systems, possibly tie into a gene that is known to be tightly controlled
			* Design theoretical circuit, then use canonical examples of reactions
			* Try the a fusion protein with the circuit that is tightly regulated in paper as a crutch
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2010-04-30T06:51:25Z

Consistent Gene Expression Level (ES)

← Older revision		Revision as of 06:51, 30 April 2010
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	==== Technical Challenges ====		==== Technical Challenges ====
	* Might have issues designing an ACR circuit synthetically		* Might have issues designing an ACR circuit synthetically
			* Getting an inducible circuit might be difficult, may start with the simpler problem of just expressing a protein at a constant level
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2010-04-30T06:50:45Z

Robustness Projects

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 ==== Approach ====
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2010-04-29T23:45:12Z

Device Projects

← Older revision		Revision as of 23:45, 29 April 2010
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	* Might need to use a combination between two irreversible recombinases or a reversible recombinase and an irreversible one		* Might need to use a combination between two irreversible recombinases or a reversible recombinase and an irreversible one
	* If recognition sequences are not compatible may need to evolve recombinases to recognize different sequences that are compatible		* If recognition sequences are not compatible may need to evolve recombinases to recognize different sequences that are compatible
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	==== Objectives ====		==== Objectives ====
	* Characterize different recombinases		* Characterize different recombinases