BE 262 project, 2010 - Revision history

Murray: /* Victor run 1, Wed afternoon */

2010-09-18T02:24:24Z

Victor run 1, Wed afternoon

← Older revision		Revision as of 02:24, 18 September 2010
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	\|}		\|}
	</blockquote>		</blockquote>
	* ~~Lost~~ the non-induced data (baseline) because we aborted without saving (~~Victor SW is stupid~~)		* Though we lost the non-induced data (baseline) because we aborted without saving, but there seems to be a file with the right timestamps (use with caution)
	* 6-8 hours of induced data; cells dried up at end of run; attached file has first 23 runs (out of ~30 total)		* 6-8 hours of induced data; cells dried up at end of run; attached file has first 23 runs (out of ~30 total)
	* Run 1 files: {{bootcamp2010 data\|TetR-GFP-plasmids_Run1-uninduced_15Sep10-1504.xls\|uninduced (xls)}}, {{bootcamp2010 data\|TetR-GFP-plasmids_Run1-induced_15Sep10-2040.xls\|induced (xls; partial run)}}		* Run 1 files: {{bootcamp2010 data\|TetR-GFP-plasmids_Run1-uninduced_15Sep10-1504.xls\|uninduced (xls)}}, {{bootcamp2010 data\|TetR-GFP-plasmids_Run1-induced_15Sep10-2040.xls\|induced (xls; partial run)}}

2010-09-18T02:22:45Z

Victor run 1, Wed afternoon

← Older revision		Revision as of 02:22, 18 September 2010
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	</blockquote>		</blockquote>
	* Lost the non-induced data (baseline) because we aborted without saving (Victor SW is stupid)		* Lost the non-induced data (baseline) because we aborted without saving (Victor SW is stupid)
	* 6-8 hours of induced data; cells dried up at end of run		* 6-8 hours of induced data; cells dried up at end of run; attached file has first 23 runs (out of ~30 total)
	* Run 1 files: {{bootcamp2010 data\|TetR-GFP-plasmids_Run1-uninduced_15Sep10-1504.xls\|uninduced (xls)}}, {{bootcamp2010 data\|TetR-GFP-plasmids_Run1-induced_15Sep10-2040.xls\|induced (xls; partial run)}}		* Run 1 files: {{bootcamp2010 data\|TetR-GFP-plasmids_Run1-uninduced_15Sep10-1504.xls\|uninduced (xls)}}, {{bootcamp2010 data\|TetR-GFP-plasmids_Run1-induced_15Sep10-2040.xls\|induced (xls; partial run)}}
	* MATLAB: {{bootcamp2010 data\|ReadVictor.m\|ReadVictor.m}} - MATLAB script to read and plot plate reader data		* MATLAB: {{bootcamp2010 data\|ReadVictor.m\|ReadVictor.m}} - MATLAB script to read and plot plate reader data

2010-09-18T02:21:43Z

Victor run 1, Wed afternoon

← Older revision		Revision as of 02:21, 18 September 2010
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	* Col 12: 1A2 (LB -> M9u) - repeat from above		* Col 12: 1A2 (LB -> M9u) - repeat from above
	\|}		\|}
	* Naming: XYZ (MM -> NN ) = plasmid pSBXYZ with pTetR:GFP, grown up in MM, transferred and measured in LB with antibiotic Y (K = kan, A = amp)		* Naming: XYZ (MM -> NN ) = plasmid pSBXYZ with pTetR:GFP, grown up in media MM, transferred and measured in media NN with antibiotic Y (K = kan, A = amp)
			** M9u = Minimal media (M9), with glucose
			** M9y = Minimal media (M9), with 0.4% glycerol
	</blockquote>		</blockquote>
	* 12 different aTC concentrations: 150 ng/ml -> 0 ng/ml in 2:1 serial diluation		* 12 different aTC concentrations: 150 ng/ml -> 0 ng/ml in 2:1 serial diluation

2010-09-18T02:20:14Z

Victor run 1, Wed afternoon

← Older revision		Revision as of 02:20, 18 September 2010
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	* Col 12: 1A2 (LB -> M9u) - repeat from above		* Col 12: 1A2 (LB -> M9u) - repeat from above
	\|}		\|}
	* Naming: XYZ (MM -> NN )~~Plasmid~~ pSBXYZ with pTetR:GFP, grown up in MM, transferred and measured in LB with Y		* Naming: XYZ (MM -> NN ) = plasmid pSBXYZ with pTetR:GFP, grown up in MM, transferred and measured in LB with antibiotic Y (K = kan, A = amp)
	</blockquote>		</blockquote>
	* 12 different aTC concentrations: 150 ng/ml -> 0 ng/ml in 2:1 serial diluation		* 12 different aTC concentrations: 150 ng/ml -> 0 ng/ml in 2:1 serial diluation

2010-09-18T02:15:47Z

Victor run 1, Wed afternoon

← Older revision		Revision as of 02:15, 18 September 2010
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	* 6-8 hours of induced data; cells dried up at end of run		* 6-8 hours of induced data; cells dried up at end of run
	* Run 1 files: {{bootcamp2010 data\|TetR-GFP-plasmids_Run1-uninduced_15Sep10-1504.xls\|uninduced (xls)}}, {{bootcamp2010 data\|TetR-GFP-plasmids_Run1-induced_15Sep10-2040.xls\|induced (xls; partial run)}}		* Run 1 files: {{bootcamp2010 data\|TetR-GFP-plasmids_Run1-uninduced_15Sep10-1504.xls\|uninduced (xls)}}, {{bootcamp2010 data\|TetR-GFP-plasmids_Run1-induced_15Sep10-2040.xls\|induced (xls; partial run)}}
			* MATLAB: {{bootcamp2010 data\|ReadVictor.m\|ReadVictor.m}} - MATLAB script to read and plot plate reader data
	* Note: check temperatures in this file; not sure they are 37 degC		* Note: check temperatures in this file; not sure they are 37 degC

2010-09-18T02:14:49Z

Victor run 3, Thu afternoon

← Older revision		Revision as of 02:14, 18 September 2010
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	</blockquote>		</blockquote>
	* 12 different aTC concentrations: 50 ng/ml -> 0 ng/ml in 2:1 serial diluation		* 12 different aTC concentrations: 50 ng/ml -> 0 ng/ml in 2:1 serial diluation
	* Run 3 files: {{bootcamp2010 data\|TTetR-GFP-plasmids_Run3-uninduced_16Sep10-1655.xls\|uninduced (xls)}}, {{bootcamp2010 data\|TetR-GFP-plasmids_Run3_induced_16Sep10-2355.xls\|induced (xls~~; partial~~)}}		* Run 3 files: {{bootcamp2010 data\|TTetR-GFP-plasmids_Run3-uninduced_16Sep10-1655.xls\|uninduced (xls)}}, {{bootcamp2010 data\|TetR-GFP-plasmids_Run3_induced_16Sep10-2355.xls\|induced (xls)}}

	=== MoFlow run 1, Thu afternoon ===		=== MoFlow run 1, Thu afternoon ===

2010-09-18T00:20:07Z

Victor run 3, Thu afternoon

← Older revision		Revision as of 00:20, 18 September 2010
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	</blockquote>		</blockquote>
	* 12 different aTC concentrations: 50 ng/ml -> 0 ng/ml in 2:1 serial diluation		* 12 different aTC concentrations: 50 ng/ml -> 0 ng/ml in 2:1 serial diluation
	* Run 3 files: {{bootcamp2010 data\|TTetR-GFP-plasmids_Run3-uninduced_16Sep10-1655.xls\|uninduced (xls)}}, {{bootcamp2010 data\|TetR-GFP-plasmids_Run3_induced_16Sep10-2355.xls\|induced (xls; partial ~~run~~)}}		* Run 3 files: {{bootcamp2010 data\|TTetR-GFP-plasmids_Run3-uninduced_16Sep10-1655.xls\|uninduced (xls)}}, {{bootcamp2010 data\|TetR-GFP-plasmids_Run3_induced_16Sep10-2355.xls\|induced (xls; partial)}}

	=== MoFlow run 1, Thu afternoon ===		=== MoFlow run 1, Thu afternoon ===

2010-09-18T00:19:35Z

Group

2010-09-18T00:18:24Z

Group

2010-09-18T00:17:58Z

Group