Murray Wiki - User contributions [en]

Research meetings, Jan/Feb 2016

2016-01-19T19:10:15Z

Srsanche:

Please sign up for a slot below. __NOTOC__

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=== 25 Jan 2016 (Mon) ===
* Richard in SF
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=== 26 Jan 2016 (Tue) ===
* 1-2 pm: Andrey Shur
* 2-3 pm: Anders Knight
* 3-4 pm: Reed McCardell
* 5:30-6:30 pm: Ania Baetica
|

=== 27 Jan 2016 (Wed) ===
* 8:30-9:30 am: Vipul Singhal
* 9:30-10:30 am: Anandh Swaminathan
* 4-5 pm: Shaobin
* 5-6 pm: Ivan (flexible)
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=== 28 Jan 2016 (Thu) ===
* 5:30-6:30 pm: Hold: Michele Colledanchise
* 6:30-7:30 pm: open
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=== 29 Jan 2016 (Fri) ===
* 2-3 pm: Victoria Hsiao
* 3-4 pm: Clare
* 4:30-5:30 pm: Ioannis Filippidis
* 5:30-6:30 pm: George Artavanis
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=== 25 Jan 2016 (Mon) ===
* Richard in Hartford
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=== 2 Feb 2016 (Tue) ===
* 9-10 am: Yong W.
* 2-3 pm: Tony Fragoso
* 3-4 pm: Sean Sanchez
* 4-5 pm: James Parkin
* 5:30-6:30 pm: Daniel Naftalovich
* 6:30-7:30 pm: Anu Thubagere
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=== 3 Feb 2016 (Wed) ===
* 8:30-9:30 am: open
* 9:30-10:30 am: open
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=== 4 Feb 2016 (Thu) ===
* Richard in SF
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=== 5 Feb 2016 (Fri) ===
* BE visiting day
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SURF discussions, Jan 2015

2015-01-20T15:15:56Z

Srsanche: /* 21 Jan (Wed) */

Slots for talking with applicants and co-mentors about SURF projects. Please sign up for one of the slots below. All times are PST. __NOTOC__

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==== 21 Jan (Wed) ====
* 9:20: Open
* 9:50: Open
<hr>
* 14:30: Rafsan Chowdhury
<hr>
* 15:30: Open
|

==== 22 Jan (Thu) ====
* 9:00: Open
<hr>
* 17:30: Open
* 18:00: Open
|

==== 23 Jan (Fri) ====
* 10:00:Aileen Cheng
<hr>
* 14:00: Open
* 14:30: Open
<hr>
* 16:00: Open
|

==== 26 Jan (Mon) ====
* 8:00: Open
* 8:30: Open
<hr>
* 17:30: Open
* 18:00: Open
|}

The agenda for the phone call is (roughly):

# Description of the basic idea behind the project (based on applicant's understanding)
# Discussion about approaches, things to read, variations to consider, etc
# Discussion of the format of the proposal
# Questions and discussion about the process

Sebastian Maerkl, Apr 2013

2014-04-04T18:29:48Z

Srsanche:

Sebastian Maerkl will visit Caltech on 7-8 April.

=== Schedule ===

Monday
* 10 am - meeting with Richard
* 11 am - open (Zach?)
* 12 pm - lunch with Richard and Zach?
* 2 pm - Informal seminar
* 3 pm - Open discussions
* 4 pm - Individual meetings

Tuesday
* Individual meetings
* ALL demo and microfluidics discussion (Enoch/Sean) (time flexible)
* 2 pm - Yutaka (time flexible; any time in Tuesday afternoon)
* 5 pm - Done for the day

=== Seminar ===

'''In vitro Synthetic Biology (a cell-free talk)'''

Sebastian J. Maerkl<br>
École Polytechnique Fédérale de Lausanne

7 Apr (Mon), 2-3 pm<br>
111 Keck

Living cells maintain a steady state of biochemical reaction rates by exchanging energy and matter with the environment. These exchanges usually do not occur in in vitro systems, which consequently go to chemical equilibrium. This in turn has severely constrained the complexity of biological networks that can be implemented in vitro. We developed nanoliter-scale microfluidic reactors that exchange reagents at dilution rates matching those of dividing bacteria. In these reactors we achieved transcription and translation at steady state for 30 h and implemented diverse regulatory mechanisms on the transcriptional, translational, and posttranslational levels, including RNA polymerases, transcriptional repression, translational activation, and proteolysis. We constructed and implemented an in vitro genetic oscillator and mapped its phase diagram showing that steady-state conditions were necessary to produce oscillations.

One potential application of in vitro synthetic biology is rapid prototyping of genetic circuits. This in turn requires that components and systems can be transferred from in vitro to in vivo, which has not yet been demonstrated for more complex genetic circuits. I will present recent results indicating that it is possible to transfer a functional in vivo genetic circuit (the repressilator) to our in vitro environment as a first step towards closing the loop between in vitro characterization and optimization of genetic circuits and their in vivo implementation.